Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (11): 2162.

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Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis

XIE Ming-Yi1, GUO Zhen-Peng1, CHEN Yi1,2*

1. Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
2. Beijing National Laboratory of Molecular Science, Beijing 100190, China

Received:2010-04-26 Online:2010-11-10 Published:2010-11-10
Contact: CHEN Yi. E-mail: chenyi@iccas.ac.cn
Supported by:
国家自然科学基金(批准号: 90717120, 20905068)、国家“九七三”计划项目(批准号: 2007CB714504)和中国科学院知识创新工程重要方向项目(批准号: KJCX2-YW-H11)资助.

Abstract

Abstract: A capillary electrophoretic method was developed to study the interaction of gold nanoparticles(GNPs) with bovine serum albumin(BSA), capable of determining the binding constant and binding capacity. For demonstration, citric acid-protected GNPs were tried, the measured bonding constant was 28.6 L/μmol and the saturated binding capacity was 24 BSA molecules per GNP, corresponding to a surface concentration of 4.5×1012 BSA/cm2. The determination was shown to depend on the incubating pH value and the concentration of run buffer which should be optimized accordingly. It was found that the peaks of the citric acid-protected GNPs were commonly broad, overlaid with irreproducible bars, but could easily be improved by addition of a small amount of BSA. The established method is expected to be extendable to inspecting the interaction of GNPs with other biomolecules.

Key words: Capillary electrophoresis, Gold nanoparticle, Bovine serum albumin, Interaction constant

TrendMD:

XIE Ming-Yi, GUO Zhen-Peng, CHEN Yi*. Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis[J]. Chem. J. Chinese Universities, 2010, 31(11): 2162.

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Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis

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