A Flow Analysis System Based on Microfluidic Chip with Continuous Sample Introduction and Gravity-driven Flows

Abstract

Abstract: An integrated microchip analytical system with gravity-driven flow was developed, equipped a novel continuous sample introduction interface with cannula configuration, featuring high sampling throughput(80-100 samples/h), low sample and reagent consumption and long continuous working time. Stable and fast flow rate for the streams of sample and reagents in microchannel of the chip were achieved by the use of horizontally oriented reservoirs and stretched outlet tubing, respectively.The performance of the system was tested by using H₂O₂ as a model sample with chemiluminescence detection by the luminol-K₃[Fe(CN)₆]-H₂O₂ reaction, achieving a good precision of 0.3%(RSD, n=5), a carryover of 1.1% for sample changing and a detection limit of 2.0×10^-7 mol/L.

Key words: Microfluidic chip, Gravity-driven flow, Continuous sample introduction, Chemiluminescence detection

CLC Number:

O657

TrendMD:

HUANG Yan-Zhen, FANG Qun, LI Dan-Ni . A Flow Analysis System Based on Microfluidic Chip with Continuous Sample Introduction and Gravity-driven Flows[J]. Chem. J. Chinese Universities, 2004, 25(9): 1628.

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A Flow Analysis System Based on Microfluidic Chip with Continuous Sample Introduction and Gravity-driven Flows

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