Preparation and Characterization of PEGylated Chitosan/DNA Self-assemble Complex and the Research on Transfection on Hela CelLIn Vitro

Abstract

Abstract: Chitosan, as a non-viral delivery system for gene therapy, has been increasingly proposed because of its biodegradable, cationic, non-toxic, good biocompatibility.As hydrophobic polymers are usually taken up by reticuloendothelial-system(RES) and have a short residence time in blood, hydrophilic, flexibleand non-ionic polymer, methoxypolyethyleneglycol(mPEG) was grafted to the amino group of chitosan to modify the chitosan′s property in this paper.mPEG-Chitosan is obtained through three steps of esterification reactions.The structures of the activated mPEGand mPEG-Chitosan were confirmed with ¹HNMR, ¹³CNMR and Fourier transform infrared(FTIR) spectrum.The solubility of PEGy lated chitosan in water is 53.6mg/mL, which becomes dissolved in water while chitosan is undissolvable.The percentage of PEGgrafted onto the amino group of chitosan was 16.71%(molar fraction).Plasmid pEGFP-N₁was chosen as model DNA.The PEGy lated chitosan/DNA complex was prepared by using an auto-coacervation process.The transfection efficiency of the PEGy lated chitosan/DNA complex in Hela cellSIn vitro was 81% analyzed by flow cytometer.Thus, The PEGy lated chitosan could be a candidate of effective non-viral vectors for gene delivery system.

Key words: PEGylated compound, Chitosan, Self-assemble complex, Non-viral vector, Transfection in vitro

CLC Number:

O629

TrendMD:

WEI Xiao-Hong, LIANG Wen-Quan, PAN Yuan-Jiang . Preparation and Characterization of PEGylated Chitosan/DNA Self-assemble Complex and the Research on Transfection on Hela CelLIn Vitro[J]. Chem. J. Chinese Universities, 2003, 24(11): 1993.

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Preparation and Characterization of PEGylated Chitosan/DNA Self-assemble Complex and the Research on Transfection on Hela CelLIn Vitro

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