MAP-H2O2-HRP Voltammetric Enzyme linked Immunoassay for Trace Southern Bean Mosaic Virus

Chem. J. Chinese Universities ›› 2000, Vol. 21 ›› Issue (8): 1200.

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MAP-H₂O₂-HRP Voltammetric Enzyme linked Immunoassay for Trace Southern Bean Mosaic Virus

ZHANG Shu-Sheng¹, JIAO Kui¹, CHEN Hong-Yuan², WANG Man-Xia¹, ZHANG Cheng-Liang³, ZHANG Zuo-Fang³

1. Departmentof App lied Chemistry, Qingdao Instituteof Chemical Technology, Qingdao 266042, China;
2. Departmentof Chemistry, Nanjing University, Nanjing 210093, China;
3. Instituteof Plant Quarantine, Ministry of Agriculture, Beijing 100029, China

Received:1999-11-17 Online:2000-08-24 Published:2000-08-24

Abstract

Abstract: Avoltammetric enzyme-linked immunoassay based on the new system of m-aminophenol(MAP)-H₂O₂-horseradish peroxidase(HRP) has been developed and used for the detection of HRPand southern bean mosaic virus(SBMV). HRPor labelled HRPcatalyzes the oxidation reaction of MAPwith H₂O₂, the product of which produces a sensitive voltammetric peak at potential of -0.46V(vs. SCE ) in Britton-Robinson(B-R) buffer solution.By using this voltammetric peak, HRPcan be measured with a detection limit of 38×10^-9 g/Land a linear range of 10×10^-8 —10×10^-6 g/L. The detection limit for the clarified SBMVis 4.0 ng/mLand the linear range of 4.0—5000 ng/mL. The highest dilution ratio detected for the infected leaf sap is 1∶1.5×10⁵.

Key words: Southern bean mosaic virus(SBMV), Horseradish peroxidase(HRP), m-Aminophenol, Immunoassay, Voltammetry

CLC Number:

O657.1

TrendMD:

ZHANG Shu-Sheng, JIAO Kui, CHEN Hong-Yuan, WANG Man-Xia, ZHANG Cheng-Liang, ZHANG Zuo-Fang . MAP-H₂O₂-HRP Voltammetric Enzyme linked Immunoassay for Trace Southern Bean Mosaic Virus[J]. Chem. J. Chinese Universities, 2000, 21(8): 1200.

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MAP-H₂O₂-HRP Voltammetric Enzyme linked Immunoassay for Trace Southern Bean Mosaic Virus

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