Studies on the Application of Synthesized Near-IR Cyanine to the Determination of Trace Amounts of Protein

Abstract

Abstract: Anew watersoluble near-IRcyanine dye was synthesized and its structure was characterized by ¹HNMR, MS and IR spectra. The dye was used as a probe to establish a novel near-IR spectrophotometric method for the determination of trace amount of serum protein. The dye has a maximum absorption wavelength at 776nm, its absorbance decreases when it reacts with protein(for example, BSA). The reaction of the dye with BSA was carried out in 0.02mol/L HAc-NaAc buffer system(pH4.0). When 3.0×10^-6mol/Lcyanine was used, the linearity of this method was over the range of 1.00×10²-1.90×10³ng/mL by determination of HSA and globulin, and the detection limit was 50ng/mL.

Key words: Near-IR dye, Cyanine, Serum protein, Spectrophotometry

CLC Number:

O657.32

TrendMD:

ZHENG Hong, LI Dong-Hui, WU Min, XU Jin-Gou, CHEN Qiu-Ying . Studies on the Application of Synthesized Near-IR Cyanine to the Determination of Trace Amounts of Protein[J]. Chem. J. Chinese Universities, 1999, 20(4): 555.

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Studies on the Application of Synthesized Near-IR Cyanine to the Determination of Trace Amounts of Protein

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