Solvent Induced Phase Separation Extraction-Spectrophotometric Method for Determination of Angiotensin-converting Enzyme Activity

doi:10.7503/cjcu20120151

Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (11): 2417.doi: 10.7503/cjcu20120151

• Analytical Chemistry • Previous Articles Next Articles

Solvent Induced Phase Separation Extraction-Spectrophotometric Method for Determination of Angiotensin-converting Enzyme Activity

TIAN Qing-Qing^1,2, ZENG Wei-Xiu¹, ZHAO Xin¹, HOU Yu-Lan¹, CHEN Bo¹, YAO Shou-Zhuo¹

1. Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China;
2. Hunan Traditional Chinese Medical college, Zhuzhou, 412008, China

Received:2012-02-23 Online:2012-11-10 Published:2012-10-15

Abstract

Abstract:

On the basis of the classical method of the determination of angiotensin-converting enzyme (ACE) activity, the solvent induced phase separation extraction (SIPSE) method and spectrophotometry were employed for the extraction and determination of hippuric acid (HA) formed during enzymatic reaction. Captopril was used as the model for the verification of the developed method. Based on the method, a traditional chinese prescription (Gastrodia and Uncaria Decoction) and the 11 kinds of main medicinal ingredients in the prescription were measured for ACE inhibition. The results show that the SIPSE method is highly selective and efficient in extracting of HA, and the recovery of HA is (97.47±0.55)%(n=3). By necessary correction, the SIPSE-spectrophotometric method is accurate and sufficient for the direct evaluation of the ACE inhibitory activity of high-matrix samples.

Key words: Solvent induced phase separation extraction(SIPSE), Angiotensin-converting enzyme, Spectrophotometry, Activity

CLC Number:

O657

TrendMD:

TIAN Qing-Qing, ZENG Wei-Xiu, ZHAO Xin, HOU Yu-Lan, CHEN Bo, YAO Shou-Zhuo. Solvent Induced Phase Separation Extraction-Spectrophotometric Method for Determination of Angiotensin-converting Enzyme Activity[J]. Chem. J. Chinese Universities, 2012, 33(11): 2417.

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Solvent Induced Phase Separation Extraction-Spectrophotometric Method for Determination of Angiotensin-converting Enzyme Activity

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