A Novel Carbazole-based Cyanine as a Fluorescent Probe for Viscosity Detection

doi:10.7503/cjcu20120155

Abstract

Abstract:

KQ, a carbazole-based cyanine dye with aldehyde, was synthesized. Dye KQ showed lower fluorescence quantum yield and no obvious fluorescence responses to the polarity of solvents and bio-macromolecules such as nucleic acids. Dye KQ was very sensitive to the environmental viscosity and gave strong fluorescence enhancement with the increase of the viscosity. Notably, there is a linear relationship between them. The live cell imaging experiments manifested that KQ could penetrate the cell membrane easily, detect the viscosity of the cellular micro-environment and gave bright and explicit staining image of the inner membrane system of live cells.

Key words: Carbazole-based cyanine dye, Viscosity, Fluorescent probe, Live cell imaging

CLC Number:

O652

TrendMD:

LIU Fei, WU Tong, HU Ming-Ming, PENG Xiao-Jun, FAN Jiang-Li. A Novel Carbazole-based Cyanine as a Fluorescent Probe for Viscosity Detection[J]. Chem. J. Chinese Universities, 2012, 33(10): 2239.

References

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