The Suppression of Ge-132 on the Maillard Reaction of BSA by Fluorescence Spcectrum

Abstract

Abstract: The fluorescences of BSAand glycosylated BSAwere observed respectively. The λ_em of BSAwas 340 nm; while the λ_em of glycosylated BSAwas 436 nm. Because the fluorescence spectra of them were different greatly, we can observe the suppression of Ge-132 on the Maillard reaction of BSAwithout any interference of itself. It was showed that the fluorescence intensity of glycosylated BSAincreased continuously with the cultured time, Ge-132 may suppress the Maillard reaction of BSAgreatly, and the suppressing efficicency would be 32%. The key site of the Maillard reaction of BSAis free amino groups of alanine residues on Nterminal amino group, besides the ε- amino groups of intrachain Lysine residues.

Key words: Ge₁₃₂, Bovine serum albumin(BSA), Alanine, Maillard reaction, Fluorescence spectrum

CLC Number:

O657

TrendMD:

CHEN Ji, MA Gen-Xiang, LI Xiao-Jing, ZHANG Shu-Gong, LI De-Qian . The Suppression of Ge-132 on the Maillard Reaction of BSA by Fluorescence Spcectrum[J]. Chem. J. Chinese Universities, 1998, 19(12): 1937.

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The Suppression of Ge-132 on the Maillard Reaction of BSA by Fluorescence Spcectrum

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