Chem. J. Chinese Universities ›› 2016, Vol. 37 ›› Issue (11): 2092.doi: 10.7503/cjcu20160437
• Polymer Chemistry • Previous Articles Next Articles
QIAN Liwei1,*(), LI Ji2, SONG Wenqi2, HU Xiaoling2, GUAN Ping2
Received:
2016-06-17
Online:
2016-11-10
Published:
2016-09-20
Contact:
QIAN Liwei
E-mail:qianliwei@mail.nwpu.edu.cn
Supported by:
CLC Number:
TrendMD:
QIAN Liwei, LI Ji, SONG Wenqi, HU Xiaoling, GUAN Ping. Utilizing Macromolecular Chain as Functional Monomer and Crosslinker to Imprint BSA with Preserving the Structural Integrity of Template†[J]. Chem. J. Chinese Universities, 2016, 37(11): 2092.
Fig.6 Synchronous fluorescence spectra of BSA influenced by micromolecular monomers(A, C) or macromolecular chains(B, D) (A), (B) Δλ =15 nm; (C), (D) Δλ =60 nm. M and C are represented micromolecular monomers and macromolecular chain, respectively.
Sample | Km/(mL·mg-1) | Qm/(mg·g-1) | R2 |
---|---|---|---|
MIH-C-BSA | 2.79 | 91.7 | 0.9929 |
NIH-C-BSA | 0.96 | 52.6 | 0.9926 |
MIH-M-BSA | 1.65 | 78.7 | 0.9969 |
NIH-M-BSA | 0.88 | 64.1 | 0.9829 |
Table 1 Theoretical maximum capacity(Qm) and Langmuir adsorption equilibrium constant(Km) from the Langmuir model
Sample | Km/(mL·mg-1) | Qm/(mg·g-1) | R2 |
---|---|---|---|
MIH-C-BSA | 2.79 | 91.7 | 0.9929 |
NIH-C-BSA | 0.96 | 52.6 | 0.9926 |
MIH-M-BSA | 1.65 | 78.7 | 0.9969 |
NIH-M-BSA | 0.88 | 64.1 | 0.9829 |
Protein | Q(MIH-C-BSA)/ (mg·mg-1) | Q(NIH-C-BSA)/ (mg·mg-1) | IFa | βa | Q(MIH-M-BSA)/ (mg·mg-1) | Q(NIH-M-BSA)/ (mg·mg-1) | IFb | βb |
---|---|---|---|---|---|---|---|---|
BSA | 67.6 | 25.5 | 2.65 | 49.2 | 30.1 | 1.63 | ||
OVA | 19.8 | 28.9 | 0.68 | 3.86 | 25.7 | 28.2 | 0.91 | 1.79 |
Hb | 16.3 | 27.8 | 0.58 | 4.52 | 27.9 | 26.2 | 1.06 | 1.53 |
Lyz | 6.7 | 13.1 | 0.51 | 5.18 | 9.2 | 13.4 | 0.68 | 2.38 |
Table 2 Selective adsorption experiments for MIH and NIH
Protein | Q(MIH-C-BSA)/ (mg·mg-1) | Q(NIH-C-BSA)/ (mg·mg-1) | IFa | βa | Q(MIH-M-BSA)/ (mg·mg-1) | Q(NIH-M-BSA)/ (mg·mg-1) | IFb | βb |
---|---|---|---|---|---|---|---|---|
BSA | 67.6 | 25.5 | 2.65 | 49.2 | 30.1 | 1.63 | ||
OVA | 19.8 | 28.9 | 0.68 | 3.86 | 25.7 | 28.2 | 0.91 | 1.79 |
Hb | 16.3 | 27.8 | 0.58 | 4.52 | 27.9 | 26.2 | 1.06 | 1.53 |
Lyz | 6.7 | 13.1 | 0.51 | 5.18 | 9.2 | 13.4 | 0.68 | 2.38 |
Fig.10 SDS-PAGE analysis of competitive adsorption of MIH-C-BSA and MIH-M-BSALane 1: protein molecular weight marker; lane 2: the mixture solution of BSA, OVA and Lyz with each concentration of 1.0 mg/mL; lane 3: the protein eluted from MIH-C-BSA; lane 4: the protein eluted from NIH-C-BSA; lane 5: the protein eluted from MIH-M-BSA; lane 6: the protein eluted from NIH-M-BSA.
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