Fabrication of Soy Protein Isolate-soluble Soy Polysaccharide Core-shell Nanogels via Maillard Reaction and Self-assembly†

doi:10.7503/cjcu20160364

Abstract

Abstract:

A “two-step” method, which involved Maillard reaction in macromolecular crowding environment and self-assembly approach, has been adopted to fabricate the novel and safe core-shell nanogels. First, amphiphilic graft copolymers were synthesized by soluble soy polysaccharide(SSPS) covalently attaching to soy protein isolate(SPI) via Maillard wet-heating reaction. Second, the resultant conjugates(SSC) were induced by both hydrophobic and electrostatic interaction to self-assemble soy protein isolate-soluble soy polysaccharide(SPI-SSPS) nanogels. Microscopic technology indicated that the SPI-SSPS nanogels were well-separated and spherical in shape with obvious core-shell structures: the hydrophilic soy polysaccharide constituted the shell and the cross-linked soy protein constituted the core. Spectroscopy investigation revealed that the tertiary structure of protein in nanogels was changed and the non-polar groups were exposed to the surface of soy protein to develop the hydrophobic compartments in the core of SPI-SSPS nanogels, which might offer a promising potential for drugs encapsulating via hydrophobic attraction. The SPI-SSPS nanogels exhibited remarkable stability against pH and NaCl concentration change, and they were pretty stable against 120 d storage at 4 ℃. All of these valuable properties provide a great potential for practical application in the field of biomedicine.

Key words: Nanogel, Maillard reaction, Self-assembly, Hydrophobic attraction, Electrostatic interaction, Soy protein isolate, Soluble soy polysaccharide

CLC Number:

TrendMD:

FENG Jilu, QI Junru, LIU Qianru. Fabrication of Soy Protein Isolate-soluble Soy Polysaccharide Core-shell Nanogels via Maillard Reaction and Self-assembly^†[J]. Chem. J. Chinese Universities, 2016, 37(11): 1999.

Figures/Tables 10

Fig.1 Electrophoresis profiles with protein staining(A) and carbohydrate staining(B)Lane M: protein marker; lane 1: native SPI; lane 2: SPI/SSPS mixture; lane 3: SSC.

Fig.2 Intrinsic fluorescence spectra of SPI(a), SPI/SSPS mixture(b) and SSC(c)

Fig.3 Effect of pH on size(a) and PDI values(b) of the fabrication of SPI-SSPS nanogels

Fig.4 DLS results of SPI(A), SSPS(B), SSC(C) and SPI-SSPS nanogels(D)

Fig.5 AFM images of SPI(A), SSPS(B), SSC(C) and SPI-SSPS nanogels(D)

Fig.6 TEM image of SPI-SSPS nanogelsInset: size distribution of nanogels.

Fig.7 Near-ultraviolet CD spectra of SPI(a), SPI/SSPS mixture(b), SSC(c) and SPI-SSPS nanogol(d)Inset: surface hydrophobicity of different samples.

Fig.8 Dh(a) and PDI values(b) of SPI-SSPS nanogels at pH=2—10

Fig.9 Dh(a) and PDI values(b) of SPI-SSPS nanogels with different NaCl concentrationsInset: visual appearance of nanogels at different NaCl concentrations.

Fig.10 Size distribution of nanogels that stored for various times

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