Chem. J. Chinese Universities ›› 1996, Vol. 17 ›› Issue (5): 710.

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Computerized Amperometric Detection for capillary Electrophoresis

HU Sben, LI Pei-Biao, CHENG Jie-Ke   

  1. Department of Chemistry, Wuhan University, Wuhan 430072
  • Received:1995-03-22 Online:1996-05-24 Published:1996-05-24

Abstract: Acomputerized end-column amperometric detector for capillary zone electrophoresis is constructed.The microelectrode is positioned at the end of 25 μm i.d.capillary by micromanipulator directly.The electrode potential is controlled and the signal is sampled by the microcomputer.Application of the system to the separation and detection of NE, IPand CATis demonstrated.This system is also applied to investigate the field-amplified injection in capillary electrophoresis with amperometric detection and the concentration limits of 0.45~0.65nmol/L for DA, NE, Eand IPare achieved.

Key words: Capillary electrophoresis, Microelectrode, Amperometric detection, Microcomputer

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