Quantitative Determination of 5-HT by Electrochemical Current-correction Method†

doi:10.7503/cjcu20170701

Abstract

Abstract:

In this paper, the carbon fiber ultramicroelectrode(CFUME) was prepared, after cyclic voltammetry scanning in 1.0×10^-4 mol/L 5-hydroxytryptamine(5-HT), and the electrochemical behaviors of the electrode in ferricyanide and dopamine(DA) were investigated. The experimental results show that the surface of carbon fiber ultramicroelectrode has been passivated by the oxidation products of hydroxytryptamine. The carbon fiber of the deteriorated ultramicroelectrodes was burned in the faint flame to activate the surface, and the peak current ratio of the activated electrode and the new electrode in 1.0×10^-3 mol/L ferricyanide solution as the correction factor, the active area of the electrode is corrected by dividing the correction factor. The experimental results show that corrected peak current and concentration of 5-HT in the range of 1.0×10^-6—2.0×10^-4 mol/L show a good linear relationship, and the liner regression equation is I=0.2073c+0.3074, with the related coefficient of R²=0.9962. The concentration of 5-HT was determined by this method and the recovery is 98.0%—102.4%. The method is simple and reproducible, and may be used to detect 5-HT in biological sample.

Key words: Carbon fiber ultramicroelectrode, Flame-burned, Activation, Current correction method, 5-Hydroxytryptamine

CLC Number:

O657.1

TrendMD:

LI Yuan,WANG Tingting,LI Mei,GU Fei,BAO Changhao,HUANG Rongping,MA Jingfang,CHENG Han. Quantitative Determination of 5-HT by Electrochemical Current-correction Method^†[J]. Chem. J. Chinese Universities, 2018, 39(5): 876.

Figures/Tables 9

Fig.1 Voltammogram curves of clean carbon fiber ultramicroelectrode in 1×10-4 mol/L 5-HT solution Note:(A) 10 scans of cyclic voltammetry; (B) 10 scans of differential pulse voltammetry.

Table 1 Peak current attenuation levels of CFUME in 1.0×104 mol/L 5-HT solution during poisoning*

Number of scan cycles	1	2	3	4	5	6	7	8	9	10
Peak current of CV/nA	36.37	16.78	11.62	6.58	4.59	4.01	2.50	1.73	1.53	0.97
Peak current decay level of CV(%)	100.00	46.14	31.95	18.09	12.62	11.03	6.87	4.76	4.21	2.67
Peak current of DPV/nA	13.36	9.27	7.26	6.13	5.10	4.39	3.82	3.35	2.97	2.60
Peak current decay level of DPV(%)	100.00	69.39	54.34	45.88	38.17	32.86	28.59	25.08	22.23	19.46

Fig.2 Electrochemical behavior of CFUME in 1.0×10-3 mol/L potassium ferricyanide solution(A, B) and in 1.0×10-4 mol/L dopamine solution(C, D) Note:(A) and (C) Cyclic voltammograms; (B) and (D) differential pulse voltammograms. a. Before poisoning; b. after poisoning; c. after activation.

Fig.3 SEM images of a clean CFUME(A), a CFUME after poisoning(B) and a CFUME after activation(C)

Fig.4 DPV overlay chart of CFUME in 1.0×10-3 mol/L potassium ferricyanide solution

Fig.5 Differential pulse voltammograms of CFUME in 1.0×10-3 mol/L potassium ferricyanide solution(A) and in gradient concentrations of 5-HT solution(B) Note: (A) a—h: Scanning sequence in K3[Fe(CN)6] solution; (B) a—g: 1.0×10-6, 5.0×10-6, 1.0×10-5, 5.0×10-5, 1.0×10-4, 1.5×10-4 and 2.0×10-4 mol/L, respectively.

Table 2 Correction of oxidation peak current for carbon fiber ultramicroelectrode in various concentrations of 5-HT(n=5)

Electrode state	c^a/(μmol·L^-1)	I^b/nA	I^c/nA	Correction factor	I^d/nA	RSD(%)
Clean electrode	1.0	45.04	0.30	1.00	0.30	2.4
The first activation	5.0	39.52	0.96	0.88	1.10	3.1
The second activation	10.0	29.08	1.86	0.65	2.86	3.5
The third activation	50.0	29.04	7.67	0.64	11.98	4.2
The fourth activation	100.0	22.75	9.82	0.51	19.25	2.7
The fifth activation	150.0	19.78	13.66	0.44	31.05	1.9
The sixth activation	200.0	18.53	17.46	0.41	42.59	1.8

Fig.6 Relationship of oxidation peak current and concentration of 5-HT before(a) and after current correction(b)

Table 3 Determination result of 5-HT in mouse serum samples(n=5)

Sample	10⁵Initial value/(mol·L^-1)	10⁵Spiked/(mol·L^-1)	10⁵Found/(mol·L^-1)	Recovery(%)
1	1.00	0.50	1.54	102.7
2	1.00	1.00	1.91	95.5
3	1.00	1.50	2.61	104.4
4	1.00	2.00	2.93	97.7
5	1.00	3.00	4.12	103.0

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