Separation and Detection of Neurotransmitter-related Substances in Urine Sample by Capillary Electrophoresis

doi:10.7503/cjcu20130835

Abstract

Abstract:

Capillary electrophoresis(CE) method was developed to separate the mixture of monoamine neurotransmitters(serotonin, dopamine, epinephrine), neurotransmitter metabolites(homovanillic acid, 5-hydroxyl indole acetic acid, vanilmandelic acid) and neurotransmitter precursors(arginine, tyrosine). The compositions, pH and concentration of background electrolyte(BGE) were optimized to separate the standard samples, meanwhile, the possible interferences by matrix components in urine as creatinine, uric acid and acetoacetic acid were investigated. Good quantitative linearity was obtained at the BGE of Na₂B₄O₇-NaOH(pH=9.90) and ultraviolet(UV) detection(200 nm) for eight neurotransmitter-related substances, which owned the limit of detection(LOD) in a range of 0.04—7.00 μmol/L. Relative standard deviation(RSD)(n=5) of migration time and peak area were 0.09%—0.48% and 0.47%—3.34%, respectively. Eventually, the developed CE method was applied for the qualitative and quantitative analysis of arginine(Arg) and vanilmandelic acid(VMA) in urine sample. Their concentrations were respectively at (95.8±3.8) μmol/L and (44.6±3.5) μmol/L with the recovery at a range of 96.65%—104.5%.

Key words: Capillary electrophoresis, Neurotransmitter, Urine, Metabolite

CLC Number:

O657.8

TrendMD:

YANG Jiao-Mei, REN Jie, LIU Hui-Qing, WANG Yong-Le, XU Zhong-Qi. Separation and Detection of Neurotransmitter-related Substances in Urine Sample by Capillary Electrophoresis[J]. Chem. J. Chinese Universities, 2013, 34(12): 2699.

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