Simultaneous Determination of 7 Components in Functional Food for Anti-hangover and Hepatoprotection by Capillary Electrophoresis†

doi:10.7503/cjcu20150980

Abstract

Abstract:

A rapid method of micellar electrokinetic capillary electrophoresis(CE) coupled with diode array detection was developed for simultaneous determination of catechin, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, dihydromyricetin and glycyrrhizic acid in functional food for anti-hangover and hepatoprotection. The parameters of capillary electrophoresis, including buffer concentration, concentration of sodium dodecyl sulfate(SDS), volume ratio of acetonitrile, and pH of the buffer, were optimized with orthogonal design. Under the optimal analytical conditions, the peak area of each analyte and its concentration had a good correlation within the linear range(r≥0.9989). Limit of detection(LOD) and quantification(LOQ) of the method were in the range of 0.26—2.22 μg/g(S/N=3) and 0.87—7.39 μg/g(S/N=10), respectively. The intra- and inter-day relative standard deviations(RSDs) of the mixed standard solution were 1.3%—2.5% and 1.9%—3.9%, respectively. While the spiked recoveries of the analytes were 91.4%—104.9% and the RSDs of the spiked samples were 1.4%—3.2%. The method of capillary electrophoresis for determination of the 7 components in functional food for anti-hangover and hepatoprotection was proposed in this study and could achieve baseline separation for all the target components within 8 min. The results show that the method could meet the requirement for routine analysis and quality control and evaluation.

Key words: Micellar electrokinetic capillary electrophoresis, Orthogonal design, Catechin, Dihydromyricetin, Glycyrrhizic acid(Ed.: D, K)

CLC Number:

O657.8

TrendMD:

ZOU Haimin, ZHOU Chen, SUN Chengjun, LI Yongxin, YANG Xiaosong, WEN Jun, ZENG Hongyan. Simultaneous Determination of 7 Components in Functional Food for Anti-hangover and Hepatoprotection by Capillary Electrophoresis^†[J]. Chem. J. Chinese Universities, 2016, 37(7): 1276.

Figures/Tables 6

References 21

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Compound	Regression equation	Correlation coefficient	Linear range/(μg·mL^-1)	LOD/(μg·g^-1)	LOQ/(μg·g^-1)
EGC	y=3049.1x-13.5	0.9995	0.09—100	0.55	1.83
C	y=4294.2x+17.9	0.9994	0.06—100	0.34	1.13
EC	y=4892.8x+27.4	0.9991	0.05—100	0.26	0.87
EGCG	y=3343.5x-33.1	0.9993	0.12—100	0.79	2.64
ECG	y=5521.2x+22.7	0.9989	0.08—100	0.63	2.09
DMY	y=3940.3x-15.5	0.9993	0.16—100	1.00	3.32
GA	y=897.2x+11.9	0.9992	0.35—200	2.22	7.39

Compound	Regression equation	Correlation coefficient	Linear range/(μg·mL^-1)	LOD/(μg·g^-1)	LOQ/(μg·g^-1)
EGC	y=3049.1x-13.5	0.9995	0.09—100	0.55	1.83
C	y=4294.2x+17.9	0.9994	0.06—100	0.34	1.13
EC	y=4892.8x+27.4	0.9991	0.05—100	0.26	0.87
EGCG	y=3343.5x-33.1	0.9993	0.12—100	0.79	2.64
ECG	y=5521.2x+22.7	0.9989	0.08—100	0.63	2.09
DMY	y=3940.3x-15.5	0.9993	0.16—100	1.00	3.32
GA	y=897.2x+11.9	0.9992	0.35—200	2.22	7.39

Compound	Intra-day precision(RSD, %)		Inter-day precision(RSD, %)
Compound	Retention time	Area	Retention time	Area
EGC	0.41	1.3	0.92	1.9
C	0.32	1.5	1.0	2.3
EC	0.54	1.9	1.3	2.8
EGCG	0.52	2.5	1.5	3.7
ECG	0.61	2.1	1.7	3.9
DMY	0.68	2.3	1.2	3.2
GA	0.77	1.7	1.4	2.6

Compound	Intra-day precision(RSD, %)		Inter-day precision(RSD, %)
Compound	Retention time	Area	Retention time	Area
EGC	0.41	1.3	0.92	1.9
C	0.32	1.5	1.0	2.3
EC	0.54	1.9	1.3	2.8
EGCG	0.52	2.5	1.5	3.7
ECG	0.61	2.1	1.7	3.9
DMY	0.68	2.3	1.2	3.2
GA	0.77	1.7	1.4	2.6

Compound	Background/(μg·mL^-1)	Added/(μg·mL^-1)	Found/(μg·mL^-1)	Recovery(%)	RSD(%)
EGC	8.27	5.00	13.0	93.9	2.7
		10.00	17.9	95.7	2.3
		20.00	27.4	95.4	2.9
C	ND	5.00	4.66	93.1	3.2
		10.00	9.48	94.8	2.7
		20.00	19.3	96.5	2.6
EC	5.88	5.00	10.4	94.1	2.5
		10.00	15.4	97.8	2.7
		20.00	26.1	102.1	1.8
EGCG	27.23	10.00	37.0	96.4	2.2
		20.00	46.6	96.2	1.4
		40.00	69.4	104.9	1.7
ECG	10.30	5.00	14.8	93.4	3.1
		10.00	19.2	91.4	2.8
		20.00	29.6	97.5	3.0
DMY	ND	5.00	4.60	92.1	3.2
		10.00	9.35	93.5	2.6
		20.00	18.3	91.6	2.2
GA	ND	20.00	20.7	103.3	2.3
		40.00	39.2	98.1	1.8
		80.00	81.8	102.2	1.7