Enantioseparation of Underivatized D,L-Serine in Biological Matrices by Capillary Electrophoresis with Contactless Conductivity Detection†

doi:10.7503/cjcu20140109

Abstract

Abstract:

A new method for enantioseparation of underivatized D,L-serine in biological matrices by capillary electrophoresis with contactless conductivity detection using binary chiral selectors(Cu-L-Arg and HPMC) was developed.The enantiomers of D,L-serine could reach an excellent baseline separation in an uncoated fused-silica capillary(50 μm i.d.×45 cm, L_eff=40 cm) under the chosen separation conditions and using 3.2 mmol/L NaOH+0.4 mmol/L Cit+2.5 mmol/L Cu(Ac)₂+5.0 mmol/L L-Arg +15.0 mg/L HPMC(pH=9.8)as the running buffer. The calibration curve of the enantiomers showed good linearity in the range of 0.35—30 mg/L with a limit of detection(LOD) of 0.10 mg/L. Effects of several factors on resolutions, such as the composition of the running buffer, pH, separation voltage and injection method were investigated to acquire the optimum conditions. The proposed method, without derivatization, has been applied to determine the D-serine contents in rat brain tissue, and was demonstrated simple, efficient, cost-effective and reproducible.

Key words: D-Serine, Enantioseparation, Capillary electrophoresis, Contactless conductivity detection, Biological matrices sample(Ed.: N, K)

CLC Number:

O657.8

TrendMD:

WEI Yu, CHEN Yufu, ZHOU Qiang, YUAN Qiuyue, TAN Fengyu, XIE Tianyao. Enantioseparation of Underivatized D,L-Serine in Biological Matrices by Capillary Electrophoresis with Contactless Conductivity Detection^†[J]. Chem. J. Chinese Universities, 2014, 35(7): 1409.

Figures/Tables 3

Fig.1 Effects of the chiral selectors on enantioseparation resolutions of serine enantiomers (A) L-Arg; (B) Cu-L-Arg; (C) Cu-L-Arg+HPMC. Separation voltage: 13 kV; gravity injection: 20 cm×60 s; contactless conductivity detection. D: D-Serine; L: L-Serine.

Fig.2 CE-C4D electropherogram of chiral separation of serine enantiomers Conditions: 3.2 mmol/L NaOH+0.4 mmol/L Cit+2.5 mmol/L Cu(Ac)2+5.0 mmol/L L-Arg+15.0 mg/L HPMC as running buffer. Other conditions are the same as those in Fig.1.

Fig.3 CE-C4D electropherogram of D-serine in rat brain tissue sample Conditions are the same as those in Fig.2.

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