Chemiluminescence Determination of D-amino Acid Based on Immobilized Enzymes

Abstract

Abstract: D-amino acid oxidase and HRPwere separately immobilized on chitosan adsorbed on the silica gels to develop a chemiluminescent method,which was combined with two oxidation reactions of D-amino acid producing H₂O₂ catalyzed by D-amino acid oxidase and of Luminol-H₂O₂ generating CLcatalyzed by HRPtogether,also was stopped──flow technique used in this system. It has a good selectivity and sensitivity. Under optimized conditions,the detection limit is 1.8 ×10^-5g/mL,the liner range of D-amino acid concentration was from 8× 10^-8 to 1. 4×10^-6g/mL,It was practicable to determine D-amino acid in serum directly with satisfactory results.

Key words: Immobilized enzymes, CL, Chitosan, D-amino acid

TrendMD:

FENG Man-Liang, HUANG Yu-Wen, GONG Zhi-Long, ZHANG Zhu-Jun. Chemiluminescence Determination of D-amino Acid Based on Immobilized Enzymes[J]. Chem. J. Chinese Universities, 1996, 17(12): 1859.

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Chemiluminescence Determination of D-amino Acid Based on Immobilized Enzymes

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