Laser-Induced Fluorcence-Intensified Charge Coupled-Device(LIF-ICCD) Used for Detection of Capillary Electrophoresis

Abstract

Abstract: Laser-induced fluorescence-intensified charge coupled-device(LIF-ICCD)was applied as detection of capillary electrophoresis.Various effects were studied both for the data collection of frequency and sensitivity of fluorescence detection in order to arrive at the optimun conditions.Acapillary electrophoregram of 4.5 ×10^-12 mol/Lof fluorescein isothiocyanate(FITC)was obtained with the LIF-ICCD detector under the chosen conditions.

Key words: ICCD detector, Laser-induced fluorescence, Capillary electrophoresis, Fluorescein isothiocyanate(FITC)

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MA Ming-Sheng, WU Xiao-Jun, LIU Guo-Quan. Laser-Induced Fluorcence-Intensified Charge Coupled-Device(LIF-ICCD) Used for Detection of Capillary Electrophoresis[J]. Chem. J. Chinese Universities, 1996, 17(12): 1862.

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Laser-Induced Fluorcence-Intensified Charge Coupled-Device(LIF-ICCD) Used for Detection of Capillary Electrophoresis

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