Spectroelectrochemical Studies of Enzymes (Ⅰ)——Electrochemical Properties of Horseradish Peroxidase

Abstract

Abstract: The redox processes of horseradish peroxidase (HRP) and its compounds were investigated by the thin-layer spectroelectrochemical techniqueIt is shown that the direct electron transfer of the enzyme HRPon the solid electrodes can be carried out and the electrode reaction is the oxidation-state transformation of the central metal ion in prosthetic group heme rather than the reduction of disulfide group in HRPmoleculeThe standard redox potential of HRP(Fe³⁺/ Fe²⁺) couple and electrochemical kinetic parameters were measured, and the electrochemical properties of HRP compound Iwere also discussed.

Key words: Horseradish peroxidase, Biological redox process, Optically transparent thin-layer electrode (OTTLE), Spectroelectrochemistry

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WU Bao-zhang, WU Hui-huang, ZHOU Shao-min . Spectroelectrochemical Studies of Enzymes (Ⅰ)——Electrochemical Properties of Horseradish Peroxidase[J]. Chem. J. Chinese Universities, 1992, 13(2): 227.

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Spectroelectrochemical Studies of Enzymes (Ⅰ)——Electrochemical Properties of Horseradish Peroxidase

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