Detection of H2O2 at Composite Membrane of [bmim] FeCl4 and PVA Loading HRP Modified Graphene/nano Au

doi:10.7503/cjcu20121075

Abstract

Abstract:

Polyvinyl alcohol-ionic liquids(PVA-ILs) composite sol, graphenes(GS) and nano-Au were used to immobilize horseradish peroxidase(HRP). A novel hydrogen peroxide biosensor(HRP/PVA-ILs/HRP/nano Au-GS/GC) was obtained by immobilizing HRP with composite membrane of PVA-ILs on a glassy carbon(GC) electrode, which was modified with graphenes and nano Au. The experimental condition was optimized and the electrochemistry behavior of the HRP/PVA-ILs/HRP/nano Au-GS/GC biosensor in 0.1 mol/L HAc-NaAc+0.1 mol/L KCl(pH=6.5) including H₂O₂ was discussed. The response of the sensor to H₂O₂ is linear in the range of 9.55×10^-6—6.01×10^-3 mol/L with a correlation coefficient of 0.9990. The detection limit is 3.3×10^-7 mol/L. The experiments show that the biosensor exhibits high sensitivity, good reproducibility and stability and longer service life.

Key words: Biosensor, Nano-Au, Graphene, 1-Butyl-3-methylimidazolium tetrachloroferrate, Horseradish peroxidase

CLC Number:

O657

TrendMD:

QU Jian-Ying, KANG Shi-Ping, LOU Tong-Fang, DU Xue-Ping. Detection of H₂O₂ at Composite Membrane of [bmim] FeCl₄ and PVA Loading HRP Modified Graphene/nano Au[J]. Chem. J. Chinese Universities, 2013, 34(9): 2097.

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Detection of H₂O₂ at Composite Membrane of [bmim] FeCl₄ and PVA Loading HRP Modified Graphene/nano Au

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