Correlativity of Characterization for Sodium Alginate/Antarctic Krill Protein by Salt Concentration†

doi:10.7503/cjcu20170015

Abstract

Abstract:

Sodium alginate/antarctic krill protein composite material(SA/AKP) was obtained by wet spinning. The hydrogen bonds of SA/AKP composite material were analyzed by Fourier transform infrared spectroscopy(FTIR), the effects of salt concentration on the crystallinity, fluidity and morphology of the composite material were studied by XRD, rheometer and SEM. Experiment manifested the existence of intramolecular and intermolecular hydrogen bonds in SA/AKP system. The strength of intermolecular hydrogen bonds was enhanced with the increase of NaCl in the composite material. At the same time, the increase of salt concentration led to SA/AKP composite material’s crystallinity increasing, the rheological properties decreasing at first and then increasing, and mechanical properties increasing at first and then decreasing. SA/AKP fiber SEM images showed that crystallized salt was separated from the fiber, and the surface structure of the composite fiber gradually decreased and became denser and smoother.Finally, AKP was completely oriented in the SA/AKP composite system.

Key words: Sodium alginate/Antarctic krill protein composite fiber, Bleng system, Wet spinning, Salt(NaCl) concentration

CLC Number:

TrendMD:

WU Jing, GUO Jing, ZHANG Sen, GONG Yumei, ZHANG Hong. Correlativity of Characterization for Sodium Alginate/Antarctic Krill Protein by Salt Concentration^†[J]. Chem. J. Chinese Universities, 2017, 38(9): 1701.

Figures/Tables 16

Fig.1 FTIR spectra of AKP(a)and SA(b)

Fig.2 FTIR spectra of SA/AKP composite fiberc(NaCl)/(g·L-1): a. 0; b. 1; c. 2; d. 3.

Fig.3 Hydrogen bonding mechanism of sodium alginate and antarctic krill protein

Fig.4 Absorbance spectra of AKP(a), SA(b) and SA/AKP composite fiber(c)

Fig.5 Two-order derivative spectra of AKP(a), SA(b) and SA/AKP composite fiber(c)

Fig.6 Gauss fitting of SA(A) and SA/AKP[c(NaCl)=0(B), 1 g/L(C)] composite fiber a. —OH; b. OH…π; c. OH…OH; d. OH…O(etheric); e. OH annular polymer; f. OH…N.

Table 1 Fitting results of various hydrogen bond types

Sample	Hydrogen bond type		$ν ˙$ /cm^-1	Peak area	Proportion(%)
Sodium alginate	Free hydroxyl	—OH	3598	7.34	5.8
	Intermolecular hydrogen bonds	OH…π	3530	33.09	26.1
		OH…O(etheric)	3300	68.75	54.2
SA/AKP composite fiber	Intramolecular hydrogen bonds	OH…OH	3411	17.62	13.9
without salt	Free hydroxyl	—OH	3600	10.22	7.5
	Intermolecular hydrogen bonds	OH…π	3484	68.09	49.7
		OH…O(etheric)	3328	28.29	20.6
		OH…N	3129	11.46	8.4
Salt concentration of 2 g/L	Intramolecular hydrogen bonds	OH…OH	3406	5.35	3.9
SA/APK composite fiber		OH cyclic polymer	3228	13.66	9.9
	Free hydroxyl	—OH	3597	12.14	1.2
	Intermolecular hydrogen bonds	OH…π	3526	106.28	10.5
		OH…O(etheric)	3300	160.36	15.8
		OH…N	3143	121.24	11.9
	Intramolecular hydrogen bonds	OH…OH	3408	488.72	48.1
		OH cyclic polymer	3228	126.41	12.5

Table 1 Fitting results of various hydrogen bond types

Sample	Hydrogen bond type		$ν ˙$ /cm^-1	Peak area	Proportion(%)
Sodium alginate	Free hydroxyl	—OH	3598	7.34	5.8
	Intermolecular hydrogen bonds	OH…π	3530	33.09	26.1
		OH…O(etheric)	3300	68.75	54.2
SA/AKP composite fiber	Intramolecular hydrogen bonds	OH…OH	3411	17.62	13.9
without salt	Free hydroxyl	—OH	3600	10.22	7.5
	Intermolecular hydrogen bonds	OH…π	3484	68.09	49.7
		OH…O(etheric)	3328	28.29	20.6
		OH…N	3129	11.46	8.4
Salt concentration of 2 g/L	Intramolecular hydrogen bonds	OH…OH	3406	5.35	3.9
SA/APK composite fiber		OH cyclic polymer	3228	13.66	9.9
	Free hydroxyl	—OH	3597	12.14	1.2
	Intermolecular hydrogen bonds	OH…π	3526	106.28	10.5
		OH…O(etheric)	3300	160.36	15.8
		OH…N	3143	121.24	11.9
	Intramolecular hydrogen bonds	OH…OH	3408	488.72	48.1
		OH cyclic polymer	3228	126.41	12.5

Fig.7 Curves of shear rate and viscosity of SA/APK composite material with different salt concentrations c(NaCl)/(g·L-1): (A) 0; (B) 1; (C) 2; (D) 3. a. 30 ℃; b. 40 ℃; c. 50 ℃.

Fig.8 Relationship between salt concentration and viscosity of SA/APK composite materialc(NaCl)/(g·L-1): a. 0; b. 1; c. 2; d. 3.

Fig.9 Relationship between salt concentration and viscosity SA/APK composite material with different shear ratesShear rate/s-1: a. 1200; b. 1800; c. 2400.

Fig.10 XRD patterns of SA(A) and AKP(B)

Fig.11 XRD patterns of SA/AKP composite material c(NaCl)/(g·L-1): a. 0; b. 1; c. 2; d. 3.

Fig.12 SEM images of SA/APK composite fibers with different salt concentrations c(NaCl)/(g·L-1): (A) 0; (B) 1; (C) 2; (D) 3.

Fig.13 Mechanical Properties of SA/AKP composite fibers

Fig.14 Half-width of the diffraction intensity curve

Fig.15 Orientation model of AKP in SA/AKP composite fiber drawing

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