Chem. J. Chinese Universities ›› 2019, Vol. 40 ›› Issue (2): 240.doi: 10.7503/cjcu20180519
• Analytical Chemistry • Previous Articles Next Articles
JIA Hongliang, ZHAO Jianwei*(), QIN Lirong, ZHAO Min
Received:
2018-07-24
Online:
2019-02-10
Published:
2018-11-16
Contact:
ZHAO Jianwei
E-mail:zhaojw@swu.edu.cn
Supported by:
CLC Number:
TrendMD:
JIA Hongliang,ZHAO Jianwei,QIN Lirong,ZHAO Min. Uric Acid Biosensor Based on Ni Wire Modified with NiO Nanosheets†[J]. Chem. J. Chinese Universities, 2019, 40(2): 240.
Fig.4 CV curves of the prepared electrode in the different concentration of UA(A) and typical CVs of the electrode in 0.01 mol PBS solution containing 0.2 mmol UA at different scan rates(from 50 mV/s to 280 mV/s)(B)Inset of (B): plots of peak currents vs. ν1/2.
Fig.5 Typical current-time response for the prepared electrode(A) or the comparison electrode to successive additions of different concentrations of UA in a stirring PBS solution at an applied potential of 0.3 V vs. Ag/AgCl(B)Inset of (B): the linear relationship between the catalytic current and the concentration of UA.
Type of electrode | Sensitivity/ (μA·mmol-1·cm-2) | Linear range/ (μmol·L-1) | Detection limit/(μmol·L-1) | Ref. |
---|---|---|---|---|
Uricase/Au NP/c-MWCNT/Au | 440.0 | 10—800 | 10.0 | [ |
Uricase/CeO2-x/C/rGO/GCE | 284.5 | 49.8—1050.0 | 2.0 | [ |
Nafion/Uricase/ZnO NSs/Ag/Si | 129.81 | 50—2000 | 0.019 | [ |
Uricase/Ni microdiscs/NiO/ITO | 431.09 | 50—1000 | 30 | [ |
Nafion/Uricase/ZnO/Au | 89.74 | 100—590 | 25.6 | [ |
CoNi nanoparticles/C | 248.2 | 25—575 | 0.08 | [ |
Nanoporous RuO2/Au | 344.2 | 20—1000 | 9.6 | [ |
Nafion/Uricase/NiO/Ni | 821.4 | 1—900 | 0.1 | This work |
Table 1 Comparison of different electrode materials for the determination of UA
Type of electrode | Sensitivity/ (μA·mmol-1·cm-2) | Linear range/ (μmol·L-1) | Detection limit/(μmol·L-1) | Ref. |
---|---|---|---|---|
Uricase/Au NP/c-MWCNT/Au | 440.0 | 10—800 | 10.0 | [ |
Uricase/CeO2-x/C/rGO/GCE | 284.5 | 49.8—1050.0 | 2.0 | [ |
Nafion/Uricase/ZnO NSs/Ag/Si | 129.81 | 50—2000 | 0.019 | [ |
Uricase/Ni microdiscs/NiO/ITO | 431.09 | 50—1000 | 30 | [ |
Nafion/Uricase/ZnO/Au | 89.74 | 100—590 | 25.6 | [ |
CoNi nanoparticles/C | 248.2 | 25—575 | 0.08 | [ |
Nanoporous RuO2/Au | 344.2 | 20—1000 | 9.6 | [ |
Nafion/Uricase/NiO/Ni | 821.4 | 1—900 | 0.1 | This work |
Real urine sample | Concentration of UA detected/ (μmol·L-1) | Spiked UA concentration/ (μmol·L-1) | Theoretical total UA concentration/ (μmol·L-1) | Measured total UA concentration/ (μmol·L-1) | Recovery(%) |
---|---|---|---|---|---|
1 | 235.7 | 100 | 335.7 | 343.6 | 102.4 |
2 | 218.4 | 200 | 418.4 | 402.3 | 96.2 |
3 | 411.2 | 400 | 811.2 | 774.9 | 95.5 |
Table 2 Determination of UA in real urine samples using our biosensor(n=3)
Real urine sample | Concentration of UA detected/ (μmol·L-1) | Spiked UA concentration/ (μmol·L-1) | Theoretical total UA concentration/ (μmol·L-1) | Measured total UA concentration/ (μmol·L-1) | Recovery(%) |
---|---|---|---|---|---|
1 | 235.7 | 100 | 335.7 | 343.6 | 102.4 |
2 | 218.4 | 200 | 418.4 | 402.3 | 96.2 |
3 | 411.2 | 400 | 811.2 | 774.9 | 95.5 |
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