Fabrication and Characterization of (Hydroxyl camptothecin@sodium cholate)-layered Double Hydroxide Nanohybrids†

doi:10.7503/cjcu20150353

Abstract

Abstract:

(Hydroxyl camptothecin@sodium cholate)-layered double hydroxide(LDH) nanohybrids were prepared by a modified drug-coassembly method. Firstly, Hydroxyl camptothecin(HCPT) was incorporated into micelles derived from sodium cholate(SCL) which negatively charged. The negatively charged micelles intercalated HCPT@SCL-LDH nanohybrids were prepared by a co-assembly between LDH nanosheets and sodium cholate micelles with the encapsulated HCPT could keep the biological active lactone form, and the drug loading of so-obtained nanohybrid was 12.9%.(HCPT@SCL)-LDH was modified using polyethylene glycol(PEG) and carboxymethylcellulose(CMC), respectively. The results showed that the dispersity of nanohybrids was obviously improved, and PEG worked better than CMC. The PEG-(HCPT@SCL)-LDH had favorable dispersity with average size of ca. 70 nm. PEG-(HCPT@SCL)-LDH showed well controlled release property and in vitro drug release kine-tics from the nanohybrids could be fitted with the pseudo-second-order kinetic model. The diffusion of HCPT through the LDH particles played an important role in controlling the drug release.

Key words: Hydroxyl camptothecin, Layered double hydroxide, Coassemble, Nanohybrid

CLC Number:

O641.1

TrendMD:

PANG Xiujiang, LIU Yuan, CHEN Li, QUAN Zhenlan. Fabrication and Characterization of (Hydroxyl camptothecin@sodium cholate)-layered Double Hydroxide Nanohybrids^†[J]. Chem. J. Chinese Universities, 2015, 36(10): 1933.

Figures/Tables 7

Fig.1 Tyndall light scattering of LDH nanosheets(A) and HCPT@SCL-LDH(B) dispersed in aqueous

Fig.2 XRD patterns of fresh LDH(a), dried Mg2Al-LDH(b) and HCPT@SCL-LDH(c)

Fig.3 FTIR spectra of Mg2Al-LDH(a), sodium cholate(b), raw HCPT(c) and HCPT@SCL-LDH nanohybrid(d)

Table 1 Average size of HCPT@SCL-LDH treated by different concentration PEG or CMC

Modifier	Arerage size/nm
Modifier	0	0.05%	0.1%	0.2%	0.5%	1.0%
PEG	349.3	294.6	269.2	68.8	195.3	230.64
CMC	349.3	328.8	307.4	301.9	115.7	375.3

Fig.4 TEM images of HCPT@SCL-LDH(A) and PEG-HCPT@SCL-LDH(B)

Fig.5 Release profiles of PEG-HCPT@SCL-LDH in pH=7.2 PBS at 37 ℃(A) and -ln(1-Xt)-t0.65 curves(B)

Fig.6 Linear regression curves of release data for PEG-(HCPT@SCL)-LDH fitting with pseudo-first-order(A) and pseudo-second-order(B) kinetic models

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