CJCU

Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (8): 1840.doi: 10.7503/cjcu20130427

• Analytical Chemistry • Previous Articles     Next Articles

Detection of Hydroxylated Metabolites of Polycyclic Aromatic Hydrocarbons by Electrospray Ionization Ion Trap Tandem Mass Spectrometry

LI Xue1,2, FANG Xiao-Wei2, LI Yin-Ping1, CHEN Huan-Wen2   

  1. 1. Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;
    2. Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang 330013, China
  • Received:2013-05-07 Online:2013-08-10 Published:2013-07-19

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Abstract:

Hydroxylated metabolites of polycyclic aromatic hydrocarbons(OH-PAHs) are the mostly widely used biomarker of carcinogenic PAHs to assess health risk caused by exposure to PAHs. Among present strategies used for detecting OH-PAHs, electrospray ionization tandem mass spectrometry(ESI-MS2) shows advantages in both high sensitivity and selectivity; however, fragment ions cannot be obtained for some OH-PAHs(e.g. 3-OHBaP, 2-OHFlu). In this work, ESI-MS2 analysis of nine OH-PAHs(2- to 5-rings) was achieved with ion trap MS2(IT-MS2); by optimizing collision-induced dissociation conditions of activation Q(AQ) and normalized collision energy(NCE), 3-OHBaP and 2-OHFlu were effectively fragmented meanwhile fragmentation efficiencies of other seven OH-PAHs were also improved. AQ was proven to be the critical parameter for fragmenting OH-PAHs when IT-MS2 is used.

Key words: Polycyclic aromatic hydrocarbon, Biomarker, Hydroxylated metabolite of polycyclic aromatic hydrocarbon, Ion trap tandem mass spectrometry, Collision-induced dissociation

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