高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (6): 901.

• 研究论文 • 上一篇    下一篇

用四氨基铝酞菁共振光散射技术测定纳克级核酸

陈小兰1, 李东辉2, 朱庆枝1, 杨黄浩1, 郑洪1, 许金钩1   

  1. 1. 厦门大学化学系, 现代分析科学教育部重点实验室;
    2. 厦门大学抗癌研究中心, 厦门 361005
  • 收稿日期:2000-07-13 出版日期:2001-06-24 发布日期:2001-06-24
  • 通讯作者: 许金钩(1937年出生),男,教授,博士生导师,主要从事分子发光分析、生化分析研究.
  • 基金资助:

    国家自然科学基金(批准号:29775021)资助

Determination of Nucleic Acids at Nanogram Levels with Tetra-substituted Amino Aluminum Phthalocyanine by a Resonance Light-scattering Technique

CHEN Xiao-Lan1, LI Dong-Hui2, ZHU Qing-Zhi1, YANG Huang-Hao1, ZHENG Hong1, XU Jin-Gou1   

  1. 1. Department of Chemistry, the Key Laboratory of Analytical Sciences of MOE;
    2. Cancer Research Center, Xiamen University, Xiamen 361005, China
  • Received:2000-07-13 Online:2001-06-24 Published:2001-06-24

PDF (PC)

被引次数

82

摘要： 痕量核酸对四氨基铝酞菁的共振散射光产生增强作用,且增强程度与核酸浓度之间有良好的线性关系.据此建立了测定核酸的高灵敏共振散射光增强分析方法.在pH=6.0、最大散射波长400nm处,测定小牛胸腺DNA(CTDNA)、鲑鱼精子DNA(SMDNA)和酵母RNA(YeastRNA)的线性范围分别是0～250ng/mL,0～200ng/mL和0～400ng/mL,检测限分别为1.4ng/mL,1.4ng/mL和2.7ng/mL.该法简单,灵敏度高,用于实际样品中核酸含量的测定,所得结果与紫外吸收法一致.

关键词: 四氨基铝酞菁, 核酸, 共振光散射

Abstract: This is the first report of the determination of nucleic acids at nanogram levels based on their enhanced effects on the resonance light-scattering of TAAlPc at 400 nm. Under optimal conditions, the linear ranges of the calibration curves were 0—2500 ng/mLfor Calf thymus DNA(CT DNA), 0—200.0 ng/mLfor Salmon sperm DNA(SM DNA) and 0—4000 ng/mLfor Yeast RNA. The detection limits were 1.4 ng/mLfor both CT DNAand SM DNA, 2.7 ng/mLfor Yeast RNA. The relative standard deviation for the determination of 100.0 ng/mL CT DNAwas 2.1%. The measurement can be made easily on a common spectrofluorimeter. The reaction between TAAlPc and nucleic acids is completed in 2 min and the scattered light signal is stable for at least 1 h. The interference from some interesting co existing substances with the determination of DNAwas also examined. The method was applied to the determination of DNAin golden staphylococcus DNAsample, and the results were satisfactory.

Key words: Tetra substituted amino aluminum phthalocyanine, Nucleic acids, Resonance light scattering

中图分类号: 

TrendMD: