高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (6): 1289.

• 研究论文 • 上一篇    下一篇

纳升电喷雾萃取电离质谱快速测定人参皂苷

胡斌1, 越皓2, 黄科科3, 姜翠翠1, 刘淑莹2, 费强3, 陈焕文1   

  1. 1. 东华理工大学, 江西省质谱科学与仪器重点实验室, 南昌 330013;
    2. 长春中医药大学吉林省人参科学研究院, 长春 130117;
    3. 吉林大学化学学院, 长春 130012
  • 收稿日期:2010-07-09 修回日期:2010-08-30 出版日期:2011-06-10 发布日期:2011-05-10
  • 通讯作者: 黄科科;陈焕文 E-mail:huangkeke_79@yahoo.com.cn;chw8868@gmail.com
  • 基金资助:

    国际科技合作项目(批准号: 2009DFA30800)和江西省科技支撑计划项目(批准号: 105)资助.

Rapid Detection of Ginsenosides Using NanoEESI-MS

HU Bin1, YUE Hao2, HUANG Ke-Ke3*, JIANG Cui-Cui1, LIU Shu-Ying2, FEI Qiang3, CHEN Huan-Wen1*   

  1. 1. Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang 330013, China;
    2. Ginseng Science Academy of Jilin Province, Changchun University of Chinese Medicine, Changchun 130117, China;
    3. College of Chemistry, Jilin University, Changchun 130012, China
  • Received:2010-07-09 Revised:2010-08-30 Online:2011-06-10 Published:2011-05-10
  • Contact: HUANG Ke-Ke;CHEN Huan-Wen E-mail:huangkeke_79@yahoo.com.cn;chw8868@gmail.com
  • Supported by:

    国际科技合作项目(批准号: 2009DFA30800)和江西省科技支撑计划项目(批准号: 105)资助.

PDF (PC)

被引次数

24

摘要： 以自行研制的纳升电喷雾萃取电离源（NanoEESI）为基础，建立了在无需色谱分离条件下快速测定人参皂苷的质谱分析方法，用NanoEESI串联质谱分析鉴定了人参中的多种人参皂苷，并将该方法用于人参、桔梗和商陆的快速鉴定。实验表明，人参中的皂苷易于在电离过程中结合钠离子，并形成[M+Na]+正离子从而被检测分析，而桔梗和商陆等样品中不含人参皂苷成份，在串联质谱中也没有相应的特征碎片离子，从而达到鉴别中药材真伪的目的。本方法简便、快速、灵敏、特征性强、重现性好，对道地中药材快速鉴定提供了思路，具有很好的实用价值。

关键词: 纳升电喷雾萃取电离, 质谱, 人参皂苷, 快速分析

Abstract: A rapid approach to detect ginsenosides has been developed based on nano electrospray extractive ionization (NanoEESI) tandem mass spectrometry. The raw extract of ginseng roots were directly introduced to the gasless nanoEESI source for production of sodiated ions of ginsenosides, without any chromatographic separation. The signals of sinsenosides [M+Na]+ were identified by tandem mass spectrometry. The characteristic fragmentation patterns were therefore used as the molecular markers to differentiate ginseng roots from platycodon grandiflorum and phytolacca acinosa rox, where are two typical plant roots illicitly used as ginseng roots on the herb medicine market. Acceptable sensitivity, reproducibility and fast speed were obtained for the samples tested, showing that nano extractive electrospray ionization tandem mass spectrometry is a useful technique for fast screening ginseng products or other traditional Chinese medicinal materials.

Key words: NanoEESI, mass spectrometry, ginsenosides, rapid analysis

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