高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (9): 2011-2017.doi: 10.7503/cjcu20200154

• 分析化学 • 上一篇    下一篇

表面修饰探针纳升电喷雾质谱脂质组学对大型溞和蚤状溞的快速鉴别

邓洁薇1, 杨运云2, 林里3, 栾天罡1,3()   

  1. 1.广东工业大学生物医药学院, 广州 510006
    2.广东省科学院, 广东省测试分析研究所(中国广州分析测试中心), 广东省化学危害应急检测技术重点实验室, 广东省原位电离质谱分析工程技术中心, 广州 510070
    3.中山大学生命科学学院, 有害生物控制与资源利用国家重点实验室和南海生物资源开发与 利用协同创新中心, 广州 510275
  • 收稿日期:2020-03-19 出版日期:2020-09-10 发布日期:2020-09-02
  • 通讯作者: 栾天罡 E-mail:cesltg@mail.sysu.edu.cn
  • 基金资助:
    国家重点研发计划项目(批准号(2018YFD0900604);国家自然科学基金(批准号(21707171);广州市科技计划项目(201804010298)

Rapid Classification of Daphniamagna and Daphnia pulex by Surface-coated Probe Nanoelectrospray Ionization Mass Spectrometry Lipidomics

DENG Jiewei1, YANG Yunyun2, LIN Li3, LUAN Tiangang1,3()   

  1. 1.School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China
    2.Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangdong Engineering and Technology Research Center for Ambient Mass Spectrometry, Guangdong Institute of Analysis(China National Analytical Center Guangzhou), Guangdong Academy of Sciences, Guangzhou 510070, China
    3.State Key Laboratory of Biocontrol, South China Sea Bio?Resource Exploitation and Utilization Collaborative Innovation Center, School of Life Sciences, Sun Yat?Sen University, Guangzhou 510275, China
  • Received:2020-03-19 Online:2020-09-10 Published:2020-09-02
  • Contact: LUAN Tiangang E-mail:cesltg@mail.sysu.edu.cn
  • Supported by:
    Supported by the National Key Research and Development Program of China(批准号(2018YFD0900604);National Natural Science Foundation of China(批准号(21707171);Guangzhou Municipal Science and Technology Project, China(201804010298)

摘要:

采用表面修饰探针敞开式纳升电喷雾质谱技术建立了脂质组学方法, 以实现溞属(Daphnia)生物大型溞和蚤状溞的快速鉴别. 采用微尺度表面修饰探针直接从Daphnia体内萃取和富集出脂质化合物, 然后在常压敞开条件下进行纳升电喷雾质谱分析, 利用高分辨率质谱仪获得脂质指纹图谱并进行脂质种类和结构鉴定. 采用该方法获得了大型溞和蚤状溞的脂质指纹图谱, 并研究了大型溞和蚤状溞体内的脂质组成特征. 通过结合化学计量学手段实现了大型溞和蚤状溞的快速区分, 并鉴定了相关的生物标志物. 结果表明, 所建立的微萃取探针纳升电喷雾质谱脂质组学方法具有分析速度快、 灵敏度高的优点, 可用于Daphnia属生物的快速鉴定和识别.

关键词: 固相微萃取, 纳升电喷雾质谱, 微尺度, 脂质组学, 物种鉴定

Abstract:

A surface-coated probe nanoelectrospray mass spectrometry(SCP-nanoESI-MS) based lipidomics method was developed for the rapid species identification of Daphnia genus. A microscale surface-coated probe was used to extract and enrich the lipids from Daphnia, and the lipids enriched on the probe were then analyzed by nanoelectrospray ionization mass spectrometry under ambient and open-air conditions. Structural identification of the lipid species was achieved via high resolution MS analysis. By using the proposed method, the lipidomics fingerprints of D. magna and D. pulex were obtained, and the characteristics of lipid compositions from D. magna and D. pulex were also investigated. Rapid classification of D. magna and D. pulex as well as the identification of lipid markers were achieved via chemometrics. The results showed that the proposed SCP-nanoESI-MS lipidomics method has the advantages of fast analysis speed and high sensitivity, and can be used for rapid identification of Daphnia species.

Key words: Solid-phase microextraction, Nanoelectrospray ionization mass spectrometry, Microscale, Lipidomics, Species identification

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