高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (7): 1505-1512.doi: 10.7503/cjcu20200262

• 分析化学 • 上一篇    下一篇

化学裂解结合生物质谱对多肽二硫键的定位

王晓宇,晏国全,周新文*(),杨芃原*()   

  1. 复旦大学生物医学研究院, 上海 200032
  • 收稿日期:2020-05-08 出版日期:2020-07-10 发布日期:2020-06-15
  • 通讯作者: 周新文,杨芃原 E-mail:zhouxinwen@fudan.edu.cn;pyyang@fudan.edu.cn
  • 基金资助:
    上海市重大疾病蛋白质组研究专业技术服务平台(18DZ2292900)

Assignment of Polypeptide Disulfide Bonds by Chemical Cleavage and Bio-mass Spectrometry

WANG Xiaoyu,YAN Guoquan,ZHOU Xinwen*(),YANG Pengyuan*()   

  1. Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
  • Received:2020-05-08 Online:2020-07-10 Published:2020-06-15
  • Contact: Xinwen ZHOU,Pengyuan YANG E-mail:zhouxinwen@fudan.edu.cn;pyyang@fudan.edu.cn
  • Supported by:
    Professional Technical Service Platform for Critical Diseases in Shanghai, China(18DZ2292900)

摘要:

二硫键是一种与多肽及蛋白质结构和功能密切相关的化学键. 当多肽中存在多个半胱氨酸时, 形成的二硫键可能会存在多种配对方式. 快速且精准地定位多肽中多对二硫键对研究多肽的结构与功能间的关系十分重要. 本文开发了一种基于化学裂解和生物质谱的新方法, 对利那洛肽中3对二硫键进行了精准定位. 通过解析裂解后特异肽段的二级质谱图, 确定利那洛肽中3对二硫键的配对方式分别为Cys1-Cys6, Cys2-Cys10和Cys5-Cys13. 该方法为二硫键的定位研究提供了新思路.

关键词: 二硫键定位, 化学裂解, 生物质谱, 利那洛肽

Abstract:

Disulfide bridges are important chemical bonds closely related to the structure and function of peptides and proteins. There may be multiple pairs of disulfide bonds when more than two cysteines existed in a peptide. Quickly and accurately assignment is crucial for evaluating the relationship between structure and function of peptide. In this paper, a comprehensive chemical cleavage and mass spectrometry method was developed to assign three pairs of disulfide bonds in Linalotide. Linalotide disulfide bonds were Cys1-Cys6, Cys2-Cys10 and Cys5-Cys13 by mapping tandem mass spectrum of specific peptides. This method provides a new idea for the study of peptide disulfide bond location.

Key words: Assignment of disulfide bond, Chemical cleavage, Bio-mass spectrometry, Linaclotide

中图分类号: