高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (3): 431-438.doi: 10.7503/cjcu20190615

• 庆祝《高等学校化学学报》复刊40周年专栏 • 上一篇    下一篇

UPLC-QTOF-MS法研究甘油三酯作为水生环境中农药污染的潜在生物标志物

何聿1,夏倩1,王文莉1,罗芳2,陈金凤3,郭雅婷1,王建1,林振宇1,*,陈国南1,*   

  1. 1. 福州大学食品安全与生物分析教育部重点实验室
    2. 生物科学与工程学院, 福州 350116
  • 收稿日期:2019-11-27 出版日期:2020-03-10 发布日期:2020-01-07
  • 通讯作者: 林振宇,陈国南
  • 作者简介:林振宇, 男, 博士, 教授, 博士生导师, 主要从事电化学方面的研究. E-mail: zylin@fzu.edu.cn|陈国南, 男, 博士, 教授, 博士生导师, 主要从事生物分析化学方面的研究. E-mail: gnchen@fzu.edu.cn
  • 基金资助:
    国家自然科学基金(Nos.21575025);国家自然科学基金(Nos.21575027);国家自然科学基金(Nos.21705025);国家自然科学基金(Nos.91843301);福建省自然科学基金资助(Nos.2018J01687);福建省自然科学基金资助.(Nos.2018J05039)

Triglycerides as Potential Biomarkers for Pesticides Pollution in Aquatic Environment Using UPLC-QTOF-MS

HE Yu1,XIA Qian1,WANG Wenli1,LUO Fang2,CHEN Jinfeng3,GUO Yating1,WANG Jian1,LIN Zhenyu1,*,CHEN Guonan1,*   

  1. 1. Key Laboratory for Analytical Science of Food Safety and Biology, Ministry of Education, 2. College of Biological Science and Engineering, Fuzhou University, Fuzhou 350116, China
    3. China Certification & Inspection Group Fujian Co. Ltd., Fuzhou 350116, China
  • Received:2019-11-27 Online:2020-03-10 Published:2020-01-07
  • Contact: Zhenyu LIN,Guonan CHEN
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.21575025);† Supported by the National Natural Science Foundation of China(Nos.21575027);† Supported by the National Natural Science Foundation of China(Nos.21705025);† Supported by the National Natural Science Foundation of China(Nos.91843301);the Natural Science Foundation of Fujian Province, China(Nos.2018J01687);the Natural Science Foundation of Fujian Province, China(Nos.2018J05039)

摘要:

以鲫鱼为代表性研究对象, 采用超高效液相色谱-四极杆飞行时间质谱(UPLC-QTOF-MS)技术, 分别研究了3种农药(敌百虫、 高效氯氰菊酯和吡虫啉)对其体内代谢的影响. 通过Masslynx软件在MS E模式下采集数据, 使用UNIFI软件进行自动检测和数据过滤, 并将差异性物质与在线代谢组数据库进行比较, 鉴定出甘油三酯类(TGs)物质具有明显差异. 其中, 吡虫啉对TGs代谢的影响最大, 且肝脏的代谢差异比大脑更显著, 低浓度的急性暴露诱导肝脏中的TGs积累, 其含量与暴露浓度之间的关系符合Michaelis-Menten方程的增长趋势. 结果表明, TGs可在短时间内(≤2 h)灵敏地反映农药吡虫啉的低浓度(≤20 ng/mL)暴露, 可作为考察吡虫啉对水生生物毒性的潜在生物标记物, 有助于建立一种快速、 灵敏的预警方法.

关键词: 超高效液相色谱-四极杆飞行时间质谱, 甘油三酯, 生物标记物, 农药污染

Abstract:

Pesticides exert a long-term negative influence on the aquatic organism and even the aquatic environment. It is necessary to find some potential biomarkers to give insight into the pesticides pollution and provide a rapid, sensitive, early-warning method. In this study, ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry(UPLC-QTOF-MS) was applied to analyze the effect of pesticides on the metabolomics in liver and brain of Crucian carp. Three pesticides, namely trichlorfon, β-cypermethrin and imidacloprid were investigated, respectively. Data was collected by masslynx software in MS E mode, analyzed by UNIFI software for automatic detection and data filtering, compared to the online metabolome database. The metabolic difference of liver was more significant than that of the brain. The triglycerides(TGs) species were identified as the different metabolites. Among these three pesticides, imidacloprid had the most significant influence on the metabolism of TGs. The low concentration of acute exposure induced the accumulation of TGs in the liver, and the relationship between the content and the exposure concentration was in line with the growth trend of Michaelis-Menten equation. The results showed that TGs can sensitively reflect the low concentration exposure of imidacloprid pesticides(≤ 20 ng/mL) in a short period of time(≤ 2 h), which can be used as a potential biomarker to investigate the toxicity of imidacloprid to aquatic organisms, and help to establish a new method for estimation the pesticides contamination in aquatic environment.

Key words: Ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry(UPLC-QTOF-MS), Triglycerides, Biomarker, Pesticides pollution

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