蛋白质组学技术筛选与鉴定癫痫疾病的差异蛋白质

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (10): 1970.

蛋白质组学技术筛选与鉴定癫痫疾病的差异蛋白质

孙志岭1, 高觉民2, 许志洋3, 王铮4, 王富强3

1. 南京中医药大学护理学院, 南京 210046;
2. 南京中医药大学附属医院神经外科, 南京 210029;
3. 南京医科大学大型仪器中心, 南京 210029;
4. 江苏广播电视大学城市科学系, 南京 210017

收稿日期:2010-04-09 出版日期:2010-10-10 发布日期:2010-10-10
通讯作者: 孙志岭, 男, 博士, 副教授, 主要从事蛋白质组学研究. E-mail:szl@njutcm.edu.cn
基金资助:
江苏省自然科学基金(批准号: BK2009428)、江苏省高校自然科学基础研究项目(批准号: 08KJB320010)及南京中医药大学基础研究与重点培育专项基金(批准号: 10XPY04)资助.

Proteomic Screening of the Differentially Expressed Proteins Related with Epilepsy

SUN Zhi-Ling1*, GAO Jue-Min2, XU Zhi-Yang3, WANG Zheng4, WANG Fu-Qiang3

1. School of Nursing, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;
2. Department of Neurosurgery, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China;
3. Center of Instrument, Nanjing Medical University, Nanjing 210029, China;
4. Urban Science Department, Jiangsu Radio and TV University, Nanjing 210017, China

Received:2010-04-09 Online:2010-10-10 Published:2010-10-10
Contact: SUN Zhi-Ling. E-mail:szl@njutcm.edu.cn
Supported by:
江苏省自然科学基金(批准号: BK2009428)、江苏省高校自然科学基础研究项目(批准号: 08KJB320010)及南京中医药大学基础研究与重点培育专项基金(批准号: 10XPY04)资助.

摘要/Abstract

摘要： 应用蛋白质组学双向凝胶电泳(Two-dimensional gel electrophoresis, 2DE)和质谱技术, 定量分析和鉴定了癫痫组(n=3)和正常组(n=3)脑组织的差异表达蛋白, 以从蛋白质水平上揭示癫痫病的发机制. 结果表明, 凝胶图谱可辨识2500~3000个蛋白点, 对21个显著差异表达蛋白点进行质谱鉴定和SwissProt数据库检索, 得到17个癫痫差异蛋白, 其中2个蛋白在癫痫组织中表达上调, 15个蛋白表达下调. 部分蛋白与癫痫的关系属首次报道. 这些蛋白与癫痫的发生发展相关, 可能成为癫痫的分子标志物和药物治疗的靶向蛋白.

关键词: 癫痫, 双向凝胶电泳, 质谱, 蛋白质组学

Abstract: To explore the mechanism of epilepsy, the proteins that were differentially expressed in the epilepsy and normal tissue were studied. Two-dimensional gel electrophoresis was performed to separate differentially expressed proteins that were subsequently identified by mass spectrometry. Totally 2500—3000 spots were detected in samples, 21 significantly differentially expressed spots were selected, and 17 proteins were identified. Two proteins in epilepsy are up-regulated, 15 proteins are down-regulated. Part of the identified protein candidates have been reported to be associated with epilepsy. However, some of the proteins identified in current were for the first time revealed to be linked to epilepsy. The functions of these proteins, the role of them in epilepsy were discussed.

Key words: Epilepsy, Two-dimensional gel electrophoresis, Mass spectrometry, Proteomics

TrendMD:

孙志岭, 高觉民, 许志洋, 王铮, 王富强. 蛋白质组学技术筛选与鉴定癫痫疾病的差异蛋白质. 高等学校化学学报, 2010, 31(10): 1970.

SUN Zhi-Ling*, GAO Jue-Min, XU Zhi-Yang, WANG Zheng, WANG Fu-Qiang. Proteomic Screening of the Differentially Expressed Proteins Related with Epilepsy. Chem. J. Chinese Universities, 2010, 31(10): 1970.

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