基于错流过滤原理的微流控细胞分离芯片的研制

高等学校化学学报

基于错流过滤原理的微流控细胞分离芯片的研制

陈兴, 崔大付, 刘长春, 李辉, 耿照新

中国科学院电子学研究所传感技术国家重点实验室, 北京100080

收稿日期:2006-04-25 修回日期:1900-01-01 出版日期:2007-01-10 发布日期:2007-01-10
通讯作者: 崔大付

Microfluidic Cell Separation Chips Based on Crossflow Filtration

CHEN Xing, CUI Da-Fu, LIU Chang-Chun, LI Hui, GENG Zhao-Xin

State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100080, China

Received:2006-04-25 Revised:1900-01-01 Online:2007-01-10 Published:2007-01-10
Contact: CUI Da-Fu

摘要/Abstract

摘要： 首次提出并制备了一种错流过滤式细胞分离微流控芯片.

关键词: 微流控芯片, 细胞分离芯片, 错流过滤, MEMS

Abstract: In this work, two kinds of novel microfluidic cells separation chips were described on the principle of crossflow filtration by using micro-electro mechanical systems(MEMS) technology. Whole blood is a complex mixture of various cells, such as red blood cells(RBC), white blood cells(WBC) and so on. Separation and collection of different kinds of cells is the required first step for the subsequent clinical and basic research assays. One-level filtration microfluidic chips with three coiled channels spaced by two arrays of parallel microfabricated filtration barriers of post-type or dam-type along the stream, were successfully used to separate and collect RBC and WBC from whole blood via their different sizes. The effects of the dilution times and the length of separation channels with different filtration barriers on RBC separation efficiency were investigated. When the whole blood was diluted by 50 times, the RBC separation efficiency was 91.2% and the WBC separation efficiency was 27.4% by using the microfluidic chip with dam-type filtration barriers of 160 mm long. The WBC separation efficiency was two times more than that by using microfluidic chip based on the dead-end filtration principle. The second kind of microfluidic chip had multilevel filtration barriers of post-type which was used to separate and collect plasma, RBC and WBC from whole blood.

Key words: Microfluidic, Cell separation chip, Crossflow filtration, MEMS

中图分类号:

O657
TP212.3

TrendMD:

陈兴, 崔大付, 刘长春, 李辉, 耿照新 . 基于错流过滤原理的微流控细胞分离芯片的研制. 高等学校化学学报, doi: .

CHEN Xing, CUI Da-Fu, LIU Chang-Chun, LI Hui, GENG Zhao-Xin. Microfluidic Cell Separation Chips Based on Crossflow Filtration. Chem. J. Chinese Universities, doi: .

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