高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (11): 2405-2410.doi: 10.7503/cjcu20120349

• 分析化学 • 上一篇    下一篇

基于尼龙微孔薄膜的微流控芯片的制作及对葡萄糖的显色响应

杨俊1,2, 齐莉1, 马会民1, 陈义1   

  1. 1. 北京分子科学国家实验室, 活体分析化学院重点实验室, 中国科学院化学研究所, 北京 100190;
    2. 中国科学院大学, 北京 100049
  • 收稿日期:2012-04-13 出版日期:2012-11-10 发布日期:2012-10-15
  • 通讯作者: 齐莉,女,博士,副研究员,主要从事芯片/毛细管电泳技术和有机聚合物整体介质的制备与应用研究.E-mail:qili@iccas.ac.cn E-mail:qili@iccas.ac.cn
  • 基金资助:

    国家自然科学基金(批准号: 21175138, 20935005)和国家"九七三"计划项目(批准号: 2007CB714504)资助.

Fabrication of Nylon Membrane-based Microfluidic Chips and Its Application in Color Sensing of Glucose

YANG Jun1,2, QI Li1, MA Hui-Min1, CHEN Yi1   

  1. 1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2012-04-13 Online:2012-11-10 Published:2012-10-15

摘要:

以尼龙微孔薄膜为基质材料, 采用光刻法将正性紫外光刻胶转移到薄膜上形成目标微通道图形, 制作了一种新型薄膜微流控芯片. 对尼龙微孔薄膜芯片的制作工艺进行了优化和改进, 使其制作时间控制在1 h内, 且制作步骤大为简化, 可实现快速、 批量地制作尼龙薄膜芯片. 在该芯片上进一步固定葡萄糖氧化酶和辣根过氧化物酶后, 利用二步酶促化学反应开展了葡萄糖的显色响应研究. 结果表明, 所制作的尼龙薄膜芯片对不同浓度的葡萄糖均能产生明显的颜色响应, 具有较宽的浓度响应范围和良好的重复性, 并可用于实际样品中葡萄糖的显色响应研究.

关键词: 尼龙薄膜, 光刻, 微流控芯片, 葡萄糖

Abstract:

A new type of nylon membrane-based microfluidic chips was fabricated by transferring the positive photoresist onto the thin membrane material via photolithography. It was found that the fabrication period can be controlled in 1 h with substantive production, and the whole fabrication processes was simplified and improved. Meanwhile, by fixing horseradish peroxidase and glucose oxidase on the membrane chips, glucose samples were assayed at different concentrations based on the two-steps enzymatic reactions. Additionally, the obviously changed color corresponding to the gradient concentration of glucose shows that the new type membrane chips could be used extensively in practice in the future.

Key words: Nylon membrane, Photolithography, Microfluidic chip, Glucose

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