高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (6): 1327-1332.doi: 10.7503/cjcu20130237

• 分析化学 • 上一篇    下一篇

水凝胶微流控芯片的快速加工及在细胞培养检测中的应用

霍丹群1, 方可敬1, 侯长军1, 杨眉1, 法焕宝2, 黄承洪1, 罗小刚1   

  1. 1. 生物流变科学与技术教育部重点实验室, 重庆大学生物工程学院, 重庆 400044;
    2. 重庆大学化学化工学院, 重庆 400030
  • 收稿日期:2013-03-14 出版日期:2013-06-10 发布日期:2013-05-15
  • 通讯作者: 侯长军,男,博士,教授,博士生导师,主要从事卟啉生化传感器与微流控生物芯片的研究.E-mail:houcj@cqu.edu.cn E-mail:houcj@cqu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 81171414, 81271930, 81102132)、 国家科技支撑计划项目(批准号: 2012BAI19B03)、 教育部高校博士学科点基金(批准号: 20090191110030)和重庆大学大型仪器设备开放平台项目(批准号: 2012121551)资助.

Rapid Fabrication of Hydrogel Microfluidic Chip and Its Application for Cell Metabolites Detection

HUO Dan-Qun1, FANG Ke-Jing1, HOU Chang-Jun1, YANG Mei1, FA Huan-Bao2, HUANG Cheng-Hong1, LUO Xiao-Gang1   

  1. 1. Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China;
    2. College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China
  • Received:2013-03-14 Online:2013-06-10 Published:2013-05-15

摘要:

采用具有紫外光聚合性能的聚乙二醇(PEG)基水凝胶材料, 通过紫外光聚合作用快速加工双层水凝胶微流控芯片, 并验证了其对肿瘤细胞代谢液进行检测的可行性. 与传统微流控芯片材料相比, 该水凝胶芯片材料具有更好的生物相容性及可操控性, 可直接加工成形, 在生物学领域特别是细胞培养过程控制方面具有良好的应用前景. 实验结果表明, 该水凝胶微流控芯片可在微尺度空间有效模拟细胞生长环境, 并实现对细胞连续捕获后的原位培养. 将该芯片与卟啉可视阵列传感器系统结合, 经代谢特征分析可有效区分不同种类肿瘤细胞, 实现芯片细胞培养平台上的细胞代谢指纹快速可视化传感检测.

关键词: 水凝胶微流控芯片, 聚乙二醇双丙烯酸酯(PEGDA), 紫外光聚合, 肿瘤细胞代谢检测

Abstract:

Taking advantage of the ultraviolet polymerization characteristic of poly(ethylene glycol) diacrylate(PEGDA) hydrogel material, a double-layer hydrogel microfluidic chip was fabricated rapidly by photopolymerization method. Moreover, feasibility of the chip culture for tumor cell metabolism detection was validated. The hydrogel material with better biocompatibility and handleability compared to traditional microfluidic chip material could be directly processed to obtain desirable micro-structure. The experimental results showed that the proposed method could achieve the fabrication of double-layer cell culture and detection chip with short time treatment. Cells could be well captured and cultured in the hydrogel microfluidic chip with excellent activity. Chip integrated with porphyrin colorimetric sensor array system could effectively distinguish different types of tumor cell by metabolism characteristics detection. The hydrogel microfluidic chip has the potential of practicable application once large-scale preparation is accomplished.

Key words: Hydrogel microfluidic chip, Poly(ethylene glycol) diacrylate(PEGDA), UV photopolymerization, Tumor cell metabolites detection

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