高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (1): 30-36.doi: 10.7503/cjcu20130854

• 分析化学 • 上一篇    下一篇

基于表面等离子体子共振成像技术研究苏拉明和顺铂对肝癌细胞生长的抑制作用

解飞, 张莹, 金伟, 潘婷婷, 周超, 任昊, 金钦汉, 牟颖()   

  1. 浙江大学工业控制技术国家重点实验室, 智能系统与控制研究所, 分析仪器研究中心, 杭州 310058
  • 收稿日期:2013-09-02 出版日期:2014-01-10 发布日期:2013-12-05
  • 作者简介:联系人简介: 牟 颖, 女, 博士, 教授, 博士生导师, 主要从事生命科学仪器及技术方面研究. E-mail:muying@zju.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 31070772, 31270907)和浙江省科技计划项目(批准号: 2011C37029)资助

Detection of Inhibitory Effects of Suramin and Cisplatin on Hepatocellular Carcinoma Cells Using Surface Plasmon Resonance Imaging

XIE Fei, ZHANG Ying, JIN Wei, PAN Tingting, ZHOU Chao, REN Hao, JIN Qinhan, MU Ying*()   

  1. Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058
  • Received:2013-09-02 Online:2014-01-10 Published:2013-12-05
  • Contact: MU Ying E-mail:muying@zju.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.31070772, 31270907) and the Science and Technology Programs of Zhejiang Province(No.2011C37029)

摘要:

基于表面等离子体子共振成像(SPRi)技术提出了一种实时、 非标记的新型抗癌药物药效评估方法. 以聚二甲基硅氧烷(PDMS)为材料, 制作了包含微柱结构的微流控芯片作为流通反应池, 配合自行设计组装的SPRi生物传感器完成肿瘤细胞的特异性捕获及检测, 研究了苏拉明和顺铂对肝癌细胞HepG2的生长抑制作用. 同时引入辅助验证实验, 即采用常规八肽胆囊收缩素(简称CCK-8)法测定上述药物对肝癌细胞增殖的抑制作用. SPRi检测结果表明, 苏拉明和顺铂能抑制肿瘤细胞HepG2增殖并呈现剂量、 时间依赖关系.

关键词: 表面等离子体子共振成像, 微流控芯片, 八肽胆囊收缩素(CCK-8)法, 苏拉明, 顺铂

Abstract:

A rapid, label-free and novel evaluation of anti-cancer drug efficacy was proposed via surface plasmon resonance imaging(SPRi). A microfluidic chip with micro-posts was designed and fabricated in polydimethylsiloxane(PDMS) to work as a continuous flow cell. Specific entrapment and detection of tumor cells were accomplished by a homemade SPRi system with the microfluidic chip. On this basis, the inhibitory effects of suramin and cisplatin on the proliferation of HepG2 cells was investigated. Meanwhile, we used auxiliary cell counting kit-8(CCK-8) assay to examine cell viability after drug incubation. The results of the SPRi method showed that both suramin and cisplatin could inhibit the proliferation of HepG2 cells in a dose-dependent and time-dependent manner.

Key words: Surface plasmon resonance imaging, Microfluidic chip, CCK-8 assay, Suramin, Cisplatin

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