高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (01): 49-53.doi: 10.3969/j.issn.0251-0790.2012.01.008

• 分析化学 • 上一篇    下一篇

微流控细胞芯片LED诱导荧光检测微系统

李栋顺1,2, 徐溢1,2,3, 彭金兰1,2,3, 周桢1, 甘俊1,2, 王尧1,2   

  1. 1. 重庆大学化学化工学院, 重庆 400030;
    2. 重庆大学新型微纳器件与系统技术国防重点学科实验室, 重庆 400030;
    3. 重庆大学微纳系统及新材料技术国际研发中心, 重庆 400030
  • 收稿日期:2011-01-17 出版日期:2012-01-10 发布日期:2011-12-20
  • 通讯作者: 徐溢,女,博士,教授,博士生导师,主要从事集成生化微纳芯片分析系统研究.E-mail:xuyibbd@sina.com E-mail:xuyibbd@sina.com
  • 基金资助:

    全国博士学位论文作者专项资金(批准号: 200941), 国家自然科学基金(批准号: 20675089), 国家科技部"创新药物孵化基地"子课题(批准号: 2010ZX09401-306-1-5), 重庆大学研究生科技创新基金(批准号: CDJXS10-22-11-39)和中央高校基本科研业务费专项资金(批准号: CDJXS1-22-00-05)资助.

Microfluidic Cell Chip Analytical System with LED Induced Fluorescence Detector

LI Dong-Shun1,2, XU Yi1,2,3, PENG Jin-Lan1,2,3, ZHOU Zhen1, GAN Jun1,2, WANG Yao1,2   

  1. 1. Chemistry and Chemical Engineering College,Chongqing Universitiy, Chongqing 400030, China;
    2. Defense Key Disciplines Laboratory of Novel Micro-nano Devices and System Technology,Chongqing Universitiy, Chongqing 400030, China;
    3. International R & D Center of Micro-nano Systems and New Materials Technology, Chongqing Universitiy, Chongqing 400030, China
  • Received:2011-01-17 Online:2012-01-10 Published:2011-12-20
  • Contact: Yi Xu E-mail:xuyibbd@sina.com

摘要: 基于微流控细胞芯片分析技术和微机电系统(MEMS)加工技术, 设计制作了阵列式微流控细胞检测芯片, 采用自组装的顶窗型光电倍增管(PMT)和信号采集电路采集芯片微管道内流动细胞的荧光信号, 构建了一种针对低浓度细胞悬浮液的微流控细胞芯片发光二极管(LED)诱导荧光的快速检测微系统, 实现了对低浓度(≤40 Cell/mL)荧光标记的HepG2肝癌细胞悬浮液样本的定量计数和测试, 而且在血液细胞共存的条件下, 仍可以有效地对血液中少量HepG2肝癌细胞进行荧光计数和测试. 整个系统结构简单, 操作方便且检测灵敏度较高.

关键词: 微流控芯片, HepG2肝癌细胞, 细胞检测, 发光二极管(LED)诱导荧光检测微系统

Abstract: Based on micro-electro-mechanical systems technique and microfluidic cell chip analytical metho-dology, a microfluidic cell chip analytical system with light emitting diode(LED) induced fluorescence detector was constructed for monitoring cells in the suspensions with a low concentration below 40 Cell/mL. Cells passed through the micro-channels on the designed microchip, and the target cells labeled by 5- or 6-(N-succinimidyloxycarbonyl)-3',6'-O,O'-diacetylfluorescein(CFDA SE) were stimulated by the LED to produce fluorescence signals which were collected by a photomultiplier tube from the roof window and changed into electrical signals finally. The HepG2 hepatoma cells labeled by CFDA SE in samples could be detected and counted by this analytical microsystem. The designed microsystem ran effectively with the coexistence of blood cells. The whole microsystem is simple, convenient and high sensitivity.

Key words: Microfluidic chip, HepG2 hepatoma cell, Cell detection, Microsystem with light emitting diode(LED) induced fluorescence detector

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