高等学校化学学报

高等学校化学学报

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胶束毛细管电泳在线推扫技术分离检测猪肉组织中痕量喹诺酮类药物

赵燕燕1,2, 王丽娟1, 李月秋2, 韩媛媛2, 杜光玲2   

    1. 河北大学药学院, 保定 071002;
    2. 河北大学医学实验中心, 保定 071000
  • 收稿日期:2006-04-13 修回日期:1900-01-01 出版日期:2007-01-10 发布日期:2007-01-10
  • 通讯作者: 赵燕燕

On-line Sweeping Determination of Quinolones in Pork Tissue by Micellar Capillary Electrophoresis

ZHAO Yan-Yan1,2, WANG Li-Juan1, LI Yue-Qiu2, HAN Yuan-Yuan2, DU Guang-Ling2   

    1. Pharmaceutical College of Hebei University, Baoding 071002, China;
    2. Medical Department of Hebei University, Baoding 071000, China
  • Received:2006-04-13 Revised:1900-01-01 Online:2007-01-10 Published:2007-01-10
  • Contact: ZHAO Yan-Yan

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被引次数

27

摘要： 利用胶束毛细管电泳法结合在线推扫富集技术对组织中残留的痕量环丙沙星、氧氟沙星和恩诺沙星进行了检测, 弥补了毛细管电泳检测灵敏度低的缺点, 大大减化了操作过程, 为动物食品组织中残留的痕量药物检测提供了一种新的简便可靠的方法.

关键词: 胶束毛细管电泳, 在线推扫技术, 痕量, 喹诺酮类药物, 猪肉组织

Abstract: A micellar capillary electrophoresis(MCE) method based on on-line sweeping technique was developed for a determination of trace quinolones, ciprofloxacin ofloxacin and enrofloxacin in pork tissue. The pork tissue samples were deproteinized with trichloroacetic acid and in the running buffer of 20 mmol/L Na2B4O7+80 mmol/L sodium dodecyl sulfate(SDS), pH=9.6, the ciprofloxacin, ofloxacin and enrofloxacin in the sample were successfully separated and detected within 200 s, in a non-coating fused-silica capillary column(48.5 cm×75 μm i.d., effective length 40 cm) at separation voltage of 18 kV, and the injection pressure of 15×102 Pa, with UV detection at 278 nm. The detection limits of ciprofloxacin, ofloxacin and enrofloxacin were 0.007, 0.016 and 0.020 mg/L, and RSD were 2.0%, 2.4% and 2.6%, respectively. This method allows of up to 600-fold accumulation concentration of ciprofloxacin and can be used to separate and detect the residuals of trace medicines in animal food.

Key words: Micellar capillary electrophoresis(MCE), On-line sweeping technique, Trace, Quinolones, Pork tissue

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