高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (6): 1243-1251.doi: 10.7503/cjcu20200080

• 分析化学 • 上一篇    下一篇

基于侧向磁泳的微流控芯片对两种磁性纳米球的同时分选

张作然,张利,张志凌*()   

  1. 武汉大学化学与分子科学学院, 武汉 430072
  • 收稿日期:2020-02-17 出版日期:2020-06-10 发布日期:2020-03-17
  • 通讯作者: 张志凌 E-mail:zlzhang@whu.edu.cn
  • 基金资助:
    国家自然科学基金(21775111)

On-chip Sorting of Beads with Different Magnetic Responsiveness by Lateral Magnetophoresis

ZHANG Zuoran,ZHANG Li,ZHANG Zhiling*()   

  1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China
  • Received:2020-02-17 Online:2020-06-10 Published:2020-03-17
  • Contact: Zhiling ZHANG E-mail:zlzhang@whu.edu.cn
  • Supported by:
    † National Natural Science Foundation of China(21775111)

摘要:

基于磁性纳米球在微流控芯片上的侧向磁泳, 利用微流控芯片分选了不同磁响应性的磁球. 提出了包含磁性纳米球聚集与偏移的理论模型, 用于分析磁球在芯片上的侧向位移. 在理论分析的基础上设计了芯片系统, 使不同磁响应性的磁纳米球可以在芯片系统上依次被分选. 实验结果表明, 2种磁性纳米球的分选效率均可接受, 且实验操作简单; 磁响应性强的磁球可被完全分离, 这对于珍贵分析样品的分选很有价值. 该分选系统被成功用于同时分选样品中乙型肝炎病毒的DNA与丙型肝炎病毒的反转录DNA, 在生化分析中具有广阔的应用前景.

关键词: 微流控芯片, 磁性纳米球, 磁分选

Abstract:

Magnetic separation is widely used in biochemical analysis. The separations of multiple small targets by magnetic force still remain a challenge. To solve the problem, different types of magnetic probes coupled with small targets have to be sorted. Here, we propose a strategy to separate magnetic nano-beads(MNs) with either a strong or weak magnetic responsiveness on a chip system. The sorting principle is utilizing the lateral magnetophoresis of MNs. A theoretical model is proposed to analyze the lateral displacement of MNs including aggregation and deflection. On this basis, chips were designed to separate MNs. By adjusting the flow rates, MNs with different magnetic responsiveness were sequentially separated. The sorting efficiency is acceptable and the procedure is easy to perform. MNs with strong magnetic responsiveness can be completely separated, which is valuable for rare targets sorting. The system was applied to sort DNA from hepatitis B virus(HBV) and complementary DNA from hepatitis C virus(HCV) simultaneously. This system has the potential for wide application in biochemical analysis.

Key words: Microfluidic chip, Magnetic nanospheres, Magnetic separation

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