高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (5): 870.

• 研究论文 • 上一篇    下一篇

重组内皮抑素的质谱分析及对内皮细胞周期的影响

王群1, 倪嘉缵1,2   

  1. 1. 中国科学院长春应用化学研究所, 稀土化学与物理国家开放实验室, 长春130022;
    2. 深圳大学生命科学学院, 深圳518060
  • 收稿日期:2004-04-29 出版日期:2005-05-10 发布日期:2005-05-10
  • 通讯作者: 倪嘉缵(1932年出生),男,博士,研究员,博士生导师,中国科学院院士,从事化学生物学、稀土化学和基因工程药物研究.E-mail:wqunstar@yahoo.com.cn E-mail:wqunstar@yahoo.com.cn
  • 基金资助:

    国家自然科学基金重大项目(批准号:29890280)资助.

Mass Spectrometry Analysis of Recombinant Endostatin and Effect on Cell Cycle of Endothelial Cells

WANG Qun1, NI Jia-Zuan1,2   

  1. 1. State Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. College of Life Science, Shenzhen University, Shenzhen 518060, China
  • Received:2004-04-29 Online:2005-05-10 Published:2005-05-10

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被引次数

2

摘要： 采用质谱、圆二色谱、荧光光谱与细胞周期分析技术研究了重组内皮抑素的蛋白结构和对内皮细胞的作用机制.研究发现,以包涵体方式表达的蛋白中会有未被降解的N末端甲硫氨酸产物,重组内皮抑素在G2期阻断内皮细胞的生长.表明内皮抑素引发内皮细胞凋亡与细胞周期相关,为抗血管生成治疗肿瘤研究奠定了实验基础.

关键词: 重组内皮抑素, 质谱, 圆二色谱, 细胞周期

Abstract: The structure of recombinant endostatin and mechanism of acting on endothelial cell were studied by mass spectrometry, circular dichroism spectroscopy, fluorescence spectroscopy and cell cycle analysis technique. It was found that the recombinant protein prepared from inclusion body of E. coli may combinate the derivated protein with N-terminal Met. The mechanism by which recombinant endostatin causes G2 arrest in endothelial cells was observed, which demonstrated that the apoptosis of endothelial cell induced by endostatin was correlated with cell cycle. The results lay the foundation for the antiangiogenesis therapy of the tumor.

Key words: Recombinant endostatin, Mass spectrometry, Circular dichroism spectroscopy, Cell cycle

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