基于液芯波导原理的微流控芯片长光程光度检测系统

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (4): 610.

基于液芯波导原理的微流控芯片长光程光度检测系统

杜文斌, 方群, 方肇伦

浙江大学化学系, 微分析系统研究所, 杭州 310028

收稿日期:2003-05-06 出版日期:2004-04-24 发布日期:2004-04-24
通讯作者: 方　群(1966年出生),男,博士,教授,从事微流控分析芯片研究.E-mail:fangqun@mai.lhz.z.jcn E-mail:fangqun@mai.lhz.z.jcn
基金资助:
国家自然科学基金重大项目(批准号:20299030);教育部科学技术研究重点项目(批准号:01093)资助

A Microfluidic Chip for Absorbance Measurements with Long Optical Path-length Based on a Liquid-core Waveguide Technique

DU Wen-Bin, FANG Qun, FANG Zhao-Lun

Institute of Microanalysis System, Department of Chemistry, Zhejiang University, Hangzhou 310028, China

Received:2003-05-06 Online:2004-04-24 Published:2004-04-24

摘要/Abstract

摘要： 提出了一种基于液芯波导(Liquidcorewaveguide,LCW)原理的微流控芯片吸收光度检测系统.通过芯片与外界接口技术实现液芯波导管与芯片的耦合,建立了芯片上长光程(毫米至厘米级)吸收光度检测池.采用邻菲啉-铁(Ⅱ)显色体系验证系统分析性能,以5.5cm外覆TeflonAF液芯波导管作为检测池(检测池体积240nL)时,芯片系统的检测线性范围为0.03～50μmol/L,对邻菲啉-铁(Ⅱ)配合物的检出限为8nmol/L,检测池有效光程达1.7cm,分析精度RSD(n=5)为0.8%.

关键词: 微流控芯片, 液芯波导, 吸收光度检测

Abstract: In this work, a system for high sensitivity absorbance measurements on microfluidic chips was developed with a detection volume of 240 nL. Long optical path-length(1.7 cm effective path length) was achieved on the chip by using an integrated liquid core waveguide(LCW) silica capillary coated with Teflon AF. The performance of the system was tested by using o-phenanthroline-Fe(Ⅱ) complex as a model sample with spectrophotometric detection, achieving a detection limit of 8 nmol/L for the complex. The absorbance of samples ranging from 0.03—50 μmol/L is linear(R 2=0.999 4) and showed good repeatability with a RSD of 0.8%(n=5). The sensitivity of the chip-LCW system was a factor of 1.7 higher than conventional spectrophotometry using a 1 cm cell. The present system can be applied in continous flow or flow injection analysis systems.

Key words: Microfluidic chip, Liquid core waveguide, Absorbance detection

中图分类号:

O657

TrendMD:

杜文斌, 方群, 方肇伦. 基于液芯波导原理的微流控芯片长光程光度检测系统. 高等学校化学学报, 2004, 25(4): 610.

DU Wen-Bin, FANG Qun, FANG Zhao-Lun. A Microfluidic Chip for Absorbance Measurements with Long Optical Path-length Based on a Liquid-core Waveguide Technique. Chem. J. Chinese Universities, 2004, 25(4): 610.

参考文献

[1] Manz A.,Graber N.,Widm er H.M..Sen s.Actuato rs B[J],1990,B 1:244—248 [2] HU Qiu-Luan(胡秋娈),LI Na(李　娜),ZHAO Feng-Lin(赵凤林)et al..Chem.J.Ch inese Universities(高等学校化学学报) [J],1999,20(2):221—223 [3] CHEN Huan-Wen(陈焕文),XU Shu-Ping(徐抒平),YU Ai-Min(于爱民)et al..Chem.J.Chinese Universities(高等学校化学学报)[J],2002,23(10):1 873—1 876 [4] Cerio tti L.,Lich tenberg J.,Clém en t S.et al..Micro-TA S 2001[C],Netherlands:Kluwer Academic Publishers,2001:339—340 [5] Liang Z.H.,Ch iem N.,Ocvirk G.et al..Anal.Chem.[J],1996,68:1 040—1 046 [6] Wolk J.,Spaid M.,Jen sen M.et al..Micro-TA S 2001[C],Netherlands:Kluw er Academ ic Publishers,2001:367—368 [7] Verpoo rte E.,Manz A.,Luedi H.et al..Sen s.Actuato rs B[J],1992,B6:66—67 [8] Takao H.,Noda T.,Ash ik iM.et al..Micro-TA S 2001[C],Netherlands:Kluw er Academ ic Publishers,2001:363—364 [9] Tiggelaar R.M.,Veen stra T.T.,Sanders R.G.P.et al..Talan ta[J],2002,56:331—339 [10] Salim i-Moo savi H.,Jiang Y.T.,Lester L.et al..Electropho resis[J],2000,21:1 291—1 299 [11] CHEN Huan-Wen(陈焕文),CAO Yan-Bo(曹彦波),HANG Song-Bai(韩松柏)et al..Ch inese Journal of Analytical Chem istry(分析化学)[J],2001,29:478—483 [12] Fuw a K.,LeiW.,Fujiw ara K..Ana.lChem.[J],1984,56:1 640—1 644 [13] FANG Qun(方　群),DU Wen-Bin(杜文斌),FANG Zhao-Lun(方肇伦).Ch inese Paten t,03 114 734.8[P],2003 [14] YU Zhen-An(于振安),ZHANG Nan(张　楠).Physical Testing and Chem ical Analysis,Part B:Chemical Analysis(理化检验,化学分册)[J],1988,24:89—90 [15] YIN Xue-Feng(殷学锋),SHEN Hong(沈　宏),FANG Zhao-Lun(方肇伦).Ch inese Journal of Analytical Chem istry(分析化学) [J],2003,31:116—119 [16] Bings N.H.,Wang C.,Sk inner C.D.et al..Anal.Chem.[J],1999,71:3 292—3 296