高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (4): 614.

• 研究论文 • 上一篇    下一篇

基于SiO2纳米粒子固定辣根过氧化物酶的生物传感器

张娟, 徐静娟, 陈洪渊   

  1. 南京大学化学系, 生命分析化学实验室, 南京 210093
  • 收稿日期:2003-01-23 出版日期:2004-04-24 发布日期:2004-04-24
  • 通讯作者: 陈洪渊(1937年出生),男,教授,博士生导师,中国科学院院士,主要从事生物分析化学研究.E-mail:hychen@nju.edu.cn E-mail:hychen@nju.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:90206037,20205007,20075010)资助

A Biosensor Based on Immobilizing Horseradish Peroxidase on SiO2 Nanoparticles

ZHANG Juan, XU Jing-Juan, CHEN Hong-Yuan   

  1. Laboratory of Life Analytical Chemistry, Department of Chemistry, Nanjing University, Nanjing 210093, China
  • Received:2003-01-23 Online:2004-04-24 Published:2004-04-24

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摘要： 制备了尺寸均一的SiO2纳米粒子(60nm),并将其用于固定辣根过氧化物酶.以儿茶酚为电子媒介体制得的H2O2传感器的检测范围为1.7×10-7～1.9×10-5mol/L,检出限为8.3×10-8mol/L.达到95%稳态电流所用时间少于10s.该传感器的米氏常数为7.8μmol/L,表明所固定的酶具有较高的生物活性.

关键词: SiO2纳米粒子, 辣根过氧化物酶, 生物传感器

Abstract: Horseradish peroxidase(HRP) was immobilized on SiO2(about 60 nm) nanoparticles. In this experiment, HRP can remain a higher bioactivity with the Michealis-Menten constant of 7.8 μmol/L . By means of catechol mediator in the solution, HRP/SiO2 modified electrode displayed an excellent electrocatalytical response linearly to the reduction of H2O2 in the concentration range of 1.7×10-7 —1.9×10-5mol/L. The detection limit was 8.3×10-8mol/L. The time for steady-state current reaching 95% is less than 10 s.

Key words: SiO2 nanaparticles, HRP, Biosensor

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