高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (3): 466-469.

• 研究论文 • 上一篇    下一篇

一种新的玉米抗氧化肽的制备与结构表征

徐力1, 李相鲁1, 吴晓霞1, 王华2, 侯瑞珍3, 黄宜兵1, 张学忠1   

  1. 1. 吉林大学分子酶学工程教育部重点实验室, 长春 130023;
    2. 吉林大学中日联谊医院, 长春 130033;
    3. 长春工业大学化工学院, 长春 130012
  • 收稿日期:2003-02-17 出版日期:2004-03-24 发布日期:2004-03-24
  • 通讯作者: 张学忠(1942年出生),男,教授,博士生导师,从事生物化学与酶工程研究.E-mail;zhangxz@mai.ljlu.edu.cn E-mail:zhangxz@mai.ljlu.edu.cn
  • 基金资助:

    国家“十五”科技攻关计划(批准号;2001BA960C)资助

Preparation and Structural Characterization of a New Corn Anti-oxidative Peptide

XU Li1, LI Xiang-Lu1, WU Xiao-Xia1, WANG Hua2, HOU Rui-Zhen3, HUANG Yi-Bing1, ZHANG Xue-Zhong1   

  1. 1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130023, China;
    2. China Japan Union Hospital of Jilin University, Changchun 130033, China;
    3. College of Chemical Engineering, Changchun University of Technology. Changchun 130012, China
  • Received:2003-02-17 Online:2004-03-24 Published:2004-03-24

摘要: 用酶法在受控条件下降解玉米醇溶蛋白,对酶解产物进行分离纯化,获得一种新的抗氧化肽;对其一级结构进行了表征,氨基酸组成和顺序为Leu-Asp-Tyr-Glu;从结构上分析了其可能的抗氧化机制.

关键词: 玉米肽, 酶水解, 抗氧化性, 层析, 质谱

Abstract: A kind of new corn anti oxidative peptide was prepared Via protease hydrolysis of zein catalyzed by alcalase, an alkaline protease under a certain condition, followed by isolation and purification. The hydrolysis time was fixed on the basis of the dependence of antioxidative activity on hydrolysis degree. It was found that the hydrolysis for 4 h gave the highest antioxidative activity. The isolation and purification steps include ultrafiltration with a membrane of cutoff Mw 1 000, successive chromatography on Sephadex G-15, Amberlite CG-120(Na) ion exchange, Sephadex G-10 and preparative HPLC columns. The amino acid sequence of the antioxidative peptide was characterized to be Leu-Asp-Tyr-Glu by amino acid analysis and electrospray ion trap mass spectrometry. We also discussed the possible anti oxidative mechanism of the antioxidative peptide on the basis of its structure.

Key words: Corn peptide, Enzymatic hydrolysis, Antioxidative activity, Chromatography, Mass spectrometry

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