高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (12): 2356.

• 研究简报 • 上一篇    下一篇

含核酸碱基混合脂质体的制备与膜内分子识别

宋立明, 凌星, 李国文   

  1. 吉林大学超分子结构与材料教育部重点实验室, 长春130023
  • 收稿日期:2003-10-16 出版日期:2004-12-24 发布日期:2004-12-24
  • 基金资助:

    国家自然科学基金(批准号:20274015);吉林大学创新基金资助

Preparation of Phosphatidylcholine/Cationic Amphiphilic Coliposomes and Molecular Recognition in the Bilayer Membranes

SONG Li-Ming, LING Xing, LI Guo-Wen   

  1. Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun 130023, China
  • Received:2003-10-16 Online:2004-12-24 Published:2004-12-24

关键词: 核酸碱基, 分子识别, 双亲化合物, 混合脂质体

Abstract: An amphiphile, 9-octanyl adenine trimethylammonium bromide(OATMAB amphiphile 3), was synthesized as the receptor. The coliposomes were prepared from phosphatidylcholine and 9-octyl adenine trimethylammonium bromide by sonication of CHCl3-cast films of amphiphiles mixtures [n(PC): n(MOATMAB)=10:1] in doubly distilled water with a bath-type sonicator at 80 W for 3 h. As thymine was added into PC/MAOTMAB coliposomal solution, the absorbability of thymine at 263 nm decreased slowly with time change from 0 to 72 h. The UV-Vis spectroscopic behavior of the thymine in PC/OATMAB/thymine coliposomes(molar ratio=10:1:1) was slowly decreaced with the increase of time. The onset of a hypochromic effect can be ascribed to adenine-thymine interaction. The absorption band levels off at 45.4% of its initial value and stabilizes after several weeks.

Key words: Nucleobases, Molecular recognition, Amphiphile, Coliposomes

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