高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (12): 2176.doi: 10.7503/cjcu20170246

• 分析化学 • 上一篇    下一篇

基于氧化石墨烯和DNA量子点组装体的DNA二元逻辑检测及循环可逆设计

沈晓琴, 李智, 王刚林, 王莉, 孙权洪, 罗序成, 马楠()   

  1. 苏州大学材料与化学化工学部, 苏州 215123
  • 收稿日期:2017-04-19 出版日期:2017-12-10 发布日期:2017-11-07
  • 作者简介:联系人简介: 马楠, 男, 博士, 教授, 博士生导师, 主要从事纳米生物学方面的研究. E-mail: nan.ma@suda.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 21175147, 91313302, 21475093)、 国家“八六三”计划项目(批准号: 2014AA020518)和青年千人计划项目资助.

Logic and Reversible Dual DNA Detection Based on the Assembly of Graphene Oxide and DNA-templated Quantum Dots

SHEN Xiaoqin, LI Zhi, WANG Ganglin, WANG Li, SUN Quanhong, LUO Xucheng, MA Nan*()   

  1. College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China
  • Received:2017-04-19 Online:2017-12-10 Published:2017-11-07
  • Contact: MA Nan E-mail:nan.ma@suda.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos. 21175147, 91313302, 21475093), the National High Technology Research and Development Program of China(No.2014AA020518) and the 1000-Young Talents Plan of China.

摘要:

制备了水溶性的氧化石墨烯(GO)和以DNA为模板的CdTe量子点(P1), 通过GO与P1的π-π堆积作用组装构建了纳米生物传感器, 将其用于双目标DNA分子的逻辑检测, 实现了较高的选择性; 通过改进DNA序列, 实现了该传感器对双目标分子的可逆循环检测及重复利用. 利用原子力显微镜(AFM)、 透射电子显微镜、 电泳和荧光光谱等方法对传感器的构建和检测过程进行了表征. 该P1-GO纳米生物传感器在核酸检测等领域具有较大的应用前景.

关键词: 氧化石墨烯, DNA, 荧光量子点探针, 双分子识别, 循环检测

Abstract:

We prepared water-soluble monolayer graphene oxide(GO) and DNA-templated CdTe quantum dots(P1), which were further assembled via π-π stacking interaction to generate nano-biosensors for the logical detection of dual DNA molecules with high selectivity. Moreover, reversible cyclic dual DNA detection is realized by changing DNA sequences, which enables regeneration of the biosensors. A variety of instruments were used to characterize biosensor preparation and detection process including atomic force microscopy(AFM), transmission electron microscopy(TEM), electrophoresis, fluorescence spectroscopy and so on. The prepared nano-biosensor holds great promise for nucleic acid detection.

Key words: Graphene oxide, DNA, Fluorescent quantum dots probe, Bimolecular recognition, Cyclic detection(Ed.: N, K)

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