聚合物在荧光检测领域的应用

doi:10.7503/cjcu20150796

高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (2): 201.doi: 10.7503/cjcu20150796

聚合物在荧光检测领域的应用

宋春霞, 羊小海(), 王柯敏(), 王青, 刘剑波, 黄晋, 李文山, 黄海花, 刘卫

湖南大学化学生物传感与计量学国家重点实验室, 化学化工学院,生物纳米与分子工程湖南省重点实验室, 长沙 410082

收稿日期:2015-10-15 出版日期:2016-02-10 发布日期:2016-01-14
作者简介:联系人简介: 羊小海, 男, 博士, 教授, 博士生导师, 主要从事核酸分子探针及生物传感器的研究. E-mail:yangxiaohai@hnu.edu.cn;王柯敏, 男, 博士, 教授, 博士生导师, 主要从事纳米及分子水平上的生物分析化学研究. E-mail:kmwang@hnu.edu.cn
基金资助:
国家自然科学基金(批准号: 21190040, 21175035)和国家“九七三”计划项目(批准号: 2011CB911002)资助

Application of Polymers in Fluorescence Analysis^†

SONG Chunxia, YANG Xiaohai^*(), WANG Kemin^*(), WANG Qing, LIU Jianbo, HUANG Jin, LI Wenshan, HUANG Haihua, LIU Wei

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering,Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province,Hunan University, Changsha 410082, China

Received:2015-10-15 Online:2016-02-10 Published:2016-01-14
Contact: YANG Xiaohai,WANG Kemin E-mail:yangxiaohai@hnu.edu.cn;kmwang@hnu.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(Nos.21190044, 21175035) and the National Basic Research Program of China(No.2011CB911002)

摘要/Abstract

摘要：

聚合物是由一种或几种重复单体以共价键连接形成的大分子化合物, 它不仅能够保持单体的性质, 而且由于聚合后单体间的协同作用, 使其表现出独特的性能. 聚合物作为基础材料在荧光检测领域得到广泛应用. 聚合物通过氢键作用、亲疏水作用及范德华力等分子间相互作用, 实现了对特定目标物的选择性识别; 通过信号转换和放大功能, 可以将分子识别作用转化为荧光信号; 可以作为骨架连接多个识别单元, 通过多价结合作用等提高识别目标物的能力, 或连接不同的功能单元, 构建多功能的分子器件. 本文对聚合物在荧光检测领域的应用进行了概述.

关键词: 聚合物, 荧光检测, 分子识别, 信号转换, 探针骨架

Abstract:

Polymers are macromolecular compounds built up from single or numerous kinds of monomers. The common materials in production and living(for example protein, nucleic acid, starch, fiber, plastic and rubber, etc.) are included in this category. Owing to the unique properties after polymerization and their monomers, polymers have been extensively used in fluorescence analysis as fundamental materials: target recognition can be realized through polymers interact with target molecules through intermolecular forces(hydrogen bond, hydrophilic and hydrophobic function, and van der Waals’ force, etc.); certein polymers can transform the molecular recognition event to easily recognizable fluorescence signal, and then fluorescence signal transformation and amplification could be realized; certein polymers have been used as framework of flourescence probes, therefore the recognition capacity of fluorescence probes are improved due to multivalency effect, and multifunction devices are constructed based on the conjugation of different functional units. In this paper, the research and application of polymers in molecular recognition, signal transformation and as framework of probes were reviewed.

Key words: Polymer, Fluorescence analysis, Molecular recognition, Signal transduction, Framework of fluorescence probe

中图分类号:

O652

TrendMD:

宋春霞, 羊小海, 王柯敏, 王青, 刘剑波, 黄晋, 李文山, 黄海花, 刘卫. 聚合物在荧光检测领域的应用. 高等学校化学学报, 2016, 37(2): 201.

SONG Chunxia, YANG Xiaohai, WANG Kemin, WANG Qing, LIU Jianbo, HUANG Jin, LI Wenshan, HUANG Haihua, LIU Wei. Application of Polymers in Fluorescence Analysis^†. Chem. J. Chinese Universities, 2016, 37(2): 201.

图/表 12

Fig.1 Application of polymers in fluorescence analyses[10—12] (C) Copyright(2010) from American Chemical Society; (D) Copyright(2014) from American Chemical Society; (E) Copyright(2013) from American Chemical Society.

Fig.2 Schematic diagram of recognition mechanism of the molecular imprinting process[16] Copyright(2011) from the Royal Society of Chemistry.

Fig.3 Dummy molecularly imprinted polymers-capped CdTe quantum dots for the fluorescent sensing of 2,4,6-trinitrotoluene[19] Copyright(2013) from American Chemical Society.

Fig.4 Proposed secondary structure of the thrombin aptamer(A) and the interaction of the aptamer with human thrombin(B) [26] Copyright(1997) from Academic Press.

Fig.5 Activatable aptamer probe for contrast-enhanced in vivo cancer imaging based on cell membrane protein-triggered conformation alteration[28] Copyright(2011) from National Academy of Sciences.

Fig.6 Schematic diagram of signal transformation mechanism of the conjugated polymers[33] Copyright(2013) from American Chemical Society.

Fig.7 Direct detection of RDX vapor using a conjugated polymer network[49] Copyright(2013) from American Chemical Society.

Fig.8 Poly(thymine)-templated selective formation of fluorescent copper nanoparticles[75] Copyright(2013) from Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Fig.9 Competitive host-guest interaction(CHGI) between β-cyclodextrin polymer and pyrene-labeled probes for fluorescence analyses[88] Copyright(2015) from American Chemical Society.

Fig.10 Engineering polymeric aptamers for selective cytotoxicity[99] Copyright(2011) from American Chemical Society.

Fig.11 A smart and versatile theranostic nanomedicine platform based on nanoporphyrin[9] Copyright(2014) from Nature Publishing Group.

Fig.12 Self-assembled supramolecular nanoprobes for ratiometric fluorescence measurement of intracellular pH values[101] Copyright(2015) from American Chemical Society.

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聚合物在荧光检测领域的应用

Application of Polymers in Fluorescence Analysis^†

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聚合物在荧光检测领域的应用

Application of Polymers in Fluorescence Analysis†

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Application of Polymers in Fluorescence Analysis^†