高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (7): 1026.

• 论文 • 上一篇    下一篇

新型近红外试剂的合成及其现场二聚体与DNA作用的研究

郑洪, 李东辉, 吴敏, 陈秋影, 许金钩   

  1. 厦门大学化学系, 教育部材料与生命过程分析科学开放研究实验室, 厦门 361005
  • 收稿日期:1998-09-07 出版日期:1999-07-24 发布日期:1999-07-24
  • 通讯作者: 许金钩
  • 作者简介:郑洪,男,31岁,博士研究生.
  • 基金资助:

    国家自然科学基金(批准号:29775021)资助课题

Synthesis of a Novel Near-IR Fluorescent Reagent and Study on its in-situ Dimer's Interaction with DNA

ZHENG Hong, LI Dong-Hui, WU Min, CHEN Qiu-Ying, XU Jin-Gou    

  1. The Research Laboratory of SEDC of Analytical Science for Meterial and Life Chemistry, Department of Chemistry, Xiamen University, Xiamen, 361005
  • Received:1998-09-07 Online:1999-07-24 Published:1999-07-24

PDF (PC)

被引次数

42

摘要： 合成了一种新型近红外阴离子染料,并对其水溶液及阳离子表面活性剂CTAB存在下的吸收及荧光光谱进行了研究.结果表明,低浓度的CTAB与该近红外阴离子染料形成离子缔合物而使阴离子染料的荧光强度降低,当CTAB的浓度进一步加大时,其胶束前预聚集促使该染料形成非荧光二聚体,导致荧光急剧猝灭.当CTAB浓度大于临界浓度时,染料二聚体解离,染料单体增溶于胶团中形成高量子产率荧光体且最大发射波长从795nm红移至812nm.对该近红外阴离子染料在CTAB的分子胶束前预聚集过程中现场形成的弱荧光二聚体作为一种双螺旋DNA的荧光测定探针的可行性做了初步的研究,并探讨其作用机制.

关键词: 合成, 近红外阴离子染料, CTAB, DNA, 荧光光谱

Abstract: Anew anionic Near-IR fluorescent cyanine dye was synthesized and its absorption and fluorescence spectra in water and in the solution of cationic surfacant--hexade-cyltrimethyl-ammonium bromide(CTAB) were studied. The result s showed that the maximum excitation wavelength and emission wavelength were 765 nm and 795 nm in water, respectively. In the presence of low concentration of CTAB, the dye formed ionic associate with CTAB was made higher to form the premiceller aggregation, the fluorescence intensity decreased dramatically because of forming a low-fluorescent in situ dimeric dye. When the concentration of CTAB was above the critical micelle concentration(cmc), the dimeric dye dissociated, and the fluorescence intensity of Near-IR cyanine was restored, and the maximum emission wavelength was bathochromated from 795 nm to 812 nm. The possibility of using low-fluorescent in situ dimeric dye as a Near-IR region fluorescent probe for DNA was also studied.

Key words: Synthesis, Near-IR dye, CTAB, DNA, Absorption and fluorescent spectra

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