高等学校化学学报

高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (5): 691.

• 论文 • 上一篇    下一篇

核苷酸、聚核苷酸和核酸猝灭Tb3+-钛铁试剂络合物荧光的机理研究

赵一兵, 王冬媛, 郭祥群, 许金钩   

  1. 厦门大学化学系, 厦门, 361005
  • 收稿日期:1996-06-27 出版日期:1997-05-24 发布日期:1997-05-24
  • 通讯作者: 赵一兵, 男, 34岁, 博士, 副教授.
  • 作者简介:赵一兵, 男, 34岁, 博士, 副教授.
  • 基金资助:

    国家教育委员会博士点基金

Studies on the Mechanism of Fluorescence Quenching of Tb3+-Tiron Complex by Nucleotides, Polynucleotides and Nucleic Acids

ZHAO Yi-Bing, WANG Dong-Yuan, GUO Xiang-Qun, XU Jin-Gou   

  1. Department of Chemistry, The Research Laboratory of SEDC of Analytical Science for Material and Life Chemistry, Xiamen University, Xiamen, 361005
  • Received:1996-06-27 Online:1997-05-24 Published:1997-05-24

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摘要： 研究了核苷酸、聚核苷酸和核酸对Tb3+－钛铁试剂(TR)络合物的荧光碎灭机理,认为荧光猝灭过程是核苷酸、聚核苷酸和核酸分子中的磷酸基组分与TR竞争Tb3+离子,生成实验条件下无荧光的二元络合物的静态猝灭过程;用Tb3+－TR络合物荧光探针研究DNA嵌入剂和金属离子与DNA相互作用的实验结果说明这一机理是合理的.

关键词: Tb3+-钛铁试剂络合物, 核苷酸, 聚核苷酸, 核酸, 荧光猝灭机理

Abstract: The mechanism of fluorescence quenching of Tb3+-Trion complex by nucleotides,polynucleotides and nucleic acids has been studied.It was considered that it is a static quenching which is based on the phosphate moieties of nucleotides, polynucleotides and nucleic acids conpeting the Tb3+ ion with Tiron and forming non-fluorescent binary complex under the optimal conditions.The experimental results of nucleic acids reacting with intercalating reagent of DNAor metal ion using Tb3+-Trion complex as a fluorescence probe indicated that the mechanism is reasonable.

Key words: Tb3+-Trion complex, Nucleotides, Polynucleotides, Nucleic acids, Mechanisin of fluorescence quenching

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