高等学校化学学报

高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (4): 525.

• 论文 • 上一篇    下一篇

水溶性游离碱阳离子卟啉与核酸作用的光谱研究

黄承志, 李克安, 童沈阳   

  1. 北京大学化学与分子工程学院, 北京, 100871
  • 收稿日期:1996-06-26 出版日期:1997-05-24 发布日期:1997-05-24
  • 通讯作者: 李克安.
  • 作者简介:黄承志, 男, 31岁, 博士, 副教授, 现在西南师范大学工作
  • 基金资助:

    国家自然科学基金;国家教育委员会博士点基金

Spectroscopic Studies on the Interactions of Water-soluble Porphyrins with Nucleic Acids

HUANG Cheng-Zhi, LI Ke-An, TONG Shen-Yang   

  1. College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871
  • Received:1996-06-26 Online:1997-05-24 Published:1997-05-24

PDF (PC)

被引次数

123

摘要： 研究了meso－四(对－三甲基氨基本基)卟啉(TAPP)和meso－四(对－甲基吡啶基)卟啉(TMPyP－4)与核酸作用的电子吸收光谱、荧光光谱和共振光散射光谱的特性.电子吸收和荧光光谱研究表明,这两个水溶性阳离子卟啉与核酸的摩尔比(R)大于0.25时,核酸对卟啉的Soret带都有减色效应和荧光碎灭效应;当R＜0.25时,核酸与卟啉作用形成新的荧光复合物.共振光散射光谱研究表明,核酸使TAPP的共振光散射增强,但对TMyyP－4的共振光散射信号无影响.

关键词: meso－四(对－三甲基氨基苯基)卟啉, meso－四(对－甲基吡啶基)卟啉, 核酸, 荧光光谱, 共振光散射光谱

Abstract: This paper presents the spectroscopic features of the interactions of two por-phyrins, meso-tetrakis (4-trimethylaminoniumphenyl )porphine (TAPP) and meso-tetrakls (N-methyl-4-pyridiniumyl)porphyrin (TMpyP-4 ) with nucleic aclds.Spectra involve electron ab-sorption, fluorescence and resonance light-scattering.The studies of absorption and fluores-cence show that the two porphyrins share common features when they interact with nucleicacids.That is, when the molar ratlo of porphyrins to nuclelc acids, R>0.25, nuclelc acidshave hypochromicity and fluorescence quenching effect on the Soret bands of the two por-phyrins;when R<0.25, new fluorescent products are formed.The difference of the interac-tions of the two porphyrins with nucleic acids can be distingushed by resonance light-scatter-ing spectra.Nucleic acids can strongly enhance the intensity of resonance light-scattering ofTAPP, but not of TMpyP-4.

Key words: meso-Tetrakis(4-trimethylaminoniumphenyl)porphine, meso-Tetrakis(N-methyl-4-pyridiniumyl)porphyrin, Nucleic acids, Fluorescence spectroscopy, Resonance light-scat-tering spectroscopy

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