高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (11): 1738.

• 论文 • 上一篇    下一篇

在肽库中进行小肽筛选的初步研究

齐杰1, 方锐1, 周慧1, 李惟1, 沈家骢2   

  1. 1. 吉林大学分子生物系, 长春, 130023;
    2. 吉林大学化学系, 长春, 130023
  • 收稿日期:1996-02-01 出版日期:1996-11-24 发布日期:1996-11-24
  • 通讯作者: 齐杰,男,28岁,博士研究生.
  • 作者简介:齐杰,男,28岁,博士研究生.
  • 基金资助:

    国家自然科学基金

A Preliminary Study on the Possibility of Screening Small Peptide in Peptide Library

QI Jie1, FANG Rui1, ZHOU Hui1, LI Wei1, SHEN Jia-Cong2   

  1. 1. Department of Molecular Biology, Jilin University, Changchun, 130023;
    2. Department of Chemistry, Jilin University, Changchun, 130023
  • Received:1996-02-01 Online:1996-11-24 Published:1996-11-24

摘要: 以合成六肽DGGSAA为模型对在噬菌体肽库中进行小肽筛选做了初步研究。结果表明,含有可形成氢键和离子键残基的小肽能够在噬菌体肽库中进行筛选,并且明显提高了筛选的专一性。筛选收率由0.001%降低到0.00022%.

关键词: 肽库, 筛选, 噬菌体,

Abstract: Peptide libraries have been successfully used in the field concerning molecular interactions,such as antigen-antibody,receptor-ligand,enzyme-inhibitor and so on.These research results show that a few critical residues which can form hydrogen bond or ionic bond in proteins make a major contribution to the interactions of proteins.This paper reports the possibility of screening short peptide in phage hexa-peptide library using DGGSAA as a model.The results show that it is possible to screen the short peptide containing the residues forming hydrogen bond and ionic bond in peptide library,which improved the specificity of screening greatly and the recovery of phage reduced from 0.001 %to 0.00022%.That indicates that the interactions between short peptides is strong enough to survive the screening and it supposes a simple model to research molecular recognition with peptide libraries.

Key words: Peptide library, Screen, Phage, Peptide

TrendMD: