高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (12): 2736-2741.doi: 10.7503/cjcu20200447

• 分析化学 • 上一篇    下一篇

多肽荧光传感器检测铜离子

阿丽1, 王勇1,2()   

  1. 1.南昌大学化学学院
    2.现代分析科学江西省重点实验室, 南昌 330031
  • 收稿日期:2020-07-13 出版日期:2020-12-10 发布日期:2020-09-01
  • 通讯作者: 王勇 E-mail:wangyong@ncu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号(21864017);31960495)、 江西省科技创新平台项目(20192BCD40001);南昌大学食品科学与技术国家重点实验室基金(SKLF-KF-201810);湖南大学生物传感及化学计量学重点实验室基金(SKLCBC-2018007)

Fluorescent Peptide Sensor for Detection of Copper Ions

A Li1, WANG Yong1,2()   

  1. 1.College of Chemistry
    2.Jiangxi Province Key Laboratory of Modern Analytical Science,Nanchang University,Nanchang 330031,China
  • Received:2020-07-13 Online:2020-12-10 Published:2020-09-01
  • Contact: WANG Yong E-mail:wangyong@ncu.edu.cn

摘要:

基于铜离子对罗丹明B标记的多肽的荧光猝灭作用, 构建了一种用于铜离子检测的荧光传感器. 利用共振光散射分析、 紫外-可见吸收光谱、 荧光寿命测试和圆二色光谱研究了铜离子检测的传感机理, 发现铜离子的加入诱导多肽结构发生变化而使罗丹明B荧光团彼此靠近, 从而导致铜离子与多肽之间发生聚集诱导荧光猝灭. 实验结果表明, 该传感器检测铜离子的线性范围为5×10?4~1×10?2 μmol/L和0.1~7 μmol/L, 检出限为0.29 nmol/L, 且拥有良好的选择性, 能用于湖水样品中铜离子的检测.

关键词: 荧光传感器, 多肽, 铜离子

Abstract:

A fluorescent sensor for copper(Ⅱ) ions was developed based on the quenching of rhodamine B-labeled peptide by copper(Ⅱ). The sensing mechanism was studied using resonance light scattering, UV-Vis absorption spectroscopy, fluorescence lifetime, and circular dichroism. The results showed that copper(Ⅱ) might induced the conformational change of the peptide that make rhodamine B approach each other, resulting in the aggregation-caused quenching effect of copper(Ⅱ) and the rhodamine B-labeled peptide. Moreover, the experimental results displayed that the sensor possessed both linear ranges of 5×10?4—1×10?2 μmol/L and 0.1—7 μmol/L with a detection limit of 0.29 nmol/L. The sensor also had a high selectivity for copper(Ⅱ), and can be successfully employed for the detection of copper(Ⅱ) ions in lake water samples.

Key words: Fluorescence sensor, Peptide, Copper(Ⅱ) ion

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