高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (11): 2345-2355.doi: 10.7503/cjcu20200397

• 庆祝《高等学校化学学报》复刊40周年专栏 • 上一篇    下一篇

DNA酶: 筛选、 生物传感及展望

佟宗轩, 胡沁沁, 顾宏周()   

  1. 复旦大学附属中山医院心血管疾病研究所, 复旦大学生物医学研究院, 上海 200032
  • 收稿日期:2020-06-29 出版日期:2020-11-10 发布日期:2020-11-06
  • 通讯作者: 顾宏周 E-mail:hongzhou.gu@fudan.edu.cn
  • 基金资助:
    国家自然科学基金(批准号(21673050);91859104, 81861138004)和国家重点研发计划项目(2016YFC1306400)

Deoxyribozymes: Selection, Biosensing and Outlook

TONG Zongxuan, HU Qinqin, GU Hongzhou()   

  1. Shanghai Institute of Cardiovascular Diseases,and Institutes of Biomedical Sciences,Zhongshan Hospital,Fudan University,Shanghai 200032,China
  • Received:2020-06-29 Online:2020-11-10 Published:2020-11-06
  • Contact: GU Hongzhou E-mail:hongzhou.gu@fudan.edu.cn
  • Supported by:
    ? Supported by the National Natural Science Foundation of China(21673050);the National Key Research and Development Program of China(2016YFC1306400)

摘要:

综述了脱氧核糖核酸酶(DNA酶)的起源及分离富集策略, 对比了DNA酶与核糖核酸酶(RNA酶)及蛋白酶的相似点和不同之处, 并重点讨论了产物捕获和对冲抵消等策略对筛选获得DNA酶的独到之处; 同时系统回顾了近年来分离出的可特异感应各种金属离子或生物样本(包括细菌、 细胞等), 从而能在特定位点切割RNA底物的DNA酶探针; 阐述了DNA酶领域现存的挑战, 总结和展望了新思路和新方向.

关键词: DNA酶, DNA催化, 指数富集的配体系统进化技术(SELEX), 筛选策略

Abstract:

In this review, we briefly describe the origin of deoxyribozymes and the selection strategies for the isolation of these deoxyribonucleic acid(DNA) enzymes. We compare deoxyribozymes with ribozymes and protein enzymes. We focus on the recently developed product-capturing and cancelling selection strategies, as well as the metal-ion-specific and particular-biological-sample-sensing ribonucleic acid(RNA)-cleaving deoxyribozymes which have worked as molecular tools in many aspects. In the end, we summarize the challenges for the deoxyribozyme field that need to be tackled down in the future.

Key words: Deoxyribozymes, Catalytic DNA, Systematic evolution of ligands by exponential enrichment(SELEX), Selection strategy

中图分类号: