高等学校化学学报

高等学校化学学报 ›› 1992, Vol. 13 ›› Issue (7): 913.

• 论文 • 上一篇    下一篇

荧光素二磷酸酯的合成和荧光分光光度法分析碱性、酸性磷酸酯酶

龚国权1, 王流芳1, 周云高1, 李瀛1, 吴安心2   

  1. 1. 兰州大学化学系, 兰州, 730000;
    2. 兰州医学院
  • 收稿日期:1991-07-08 出版日期:1992-07-24 发布日期:1992-07-24
  • 通讯作者: 王流芳
  • 基金资助:

    国家自然科学基金资助课题

Synthesis of Fluorescein Diphosphate and Its Analysis of Alkaline and Acidic Phosphatase by Fluorimetry

GONG Guo-quan1, WANG Liu-fang1, ZHOU Yun-gao1, LI Ying1, WU An-xin2   

  1. 1. Department of Chemistry, Lanzhou University, Lanzhou, 730000;
    2. Lanzhou Medical College, Lanzhou
  • Received:1991-07-08 Online:1992-07-24 Published:1992-07-24

PDF (PC)

被引次数

9

摘要： 目前荧光光度法测定碱性和酸性磷酸酯酶的底物常用伞形酮磷酸酯[1]和萘酚As-BI(6-溴-3-羟基-2-萘基邻茴香胺)的磷酸二氢酯[2].它们具有很高的灵敏度,在荧光免疫分析中被广泛应用.

关键词: 荧光素二磷酸酯, 合成, 碱性和酸性磷酸酯酶, 荧光光度法

Abstract: Fluorescein diphosphate was synthesized with fluorescein and phosphorus oxychloride.The diphosphate was applied to the fluorimetric analysis of alkaline and acidic phosphatase.The conditions of reaction of phosphatase with fluorescein diphosphate, linear range for the determination of the phosphatase, limitation of detection of the phosphatase and Michaelis constant were studied.

Key words: Fluorescein diphosphate, Synthesis, Alkaline and acidic phosphatase, Fluorimetric method

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